miR156 and miR390 Regulate tasiRNA Accumulation and Developmental Timing in Physcomitrella patens
miR156 and miR390 Regulate tasiRNA Accumulation and Developmental Timing in Physcomitrella patens
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DOI：
10.1105/tpc.112.103176
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发表时间：
2012-12-01
期刊：
影响因子：
11.6
通讯作者：
Axtell, Michael J.
中科院分区：
文献类型：
--
作者：
Cho, Sung Hyun;Coruh, Ceyda;Axtell, Michael J.
MicroRNA156(MiR156)影响开花植物的发育时间。MiR156及其与鳞状病毒启动子结合蛋白(SPL)基因家族成员的靶标关系在陆地植物中普遍保守，但miR156在开花植物外的具体功能尚不清楚。我们发现miR156促进了苔藓Physcomitrella patens中从年轻的丝状原丝体到叶状配子柄的发育变化，与它作为开花植物发育抑制因子的作用相反。专利杆菌miR156也影响依赖于第二个古老的microRNA miR390的反式作用小干扰RNA(TasiRNAs)的积累。MiR156和miR390都直接针对单个主要的tasiRNA初级转录本。抑制miR156功能会导致miR390触发的tasiRNA积聚增加，并减少tasiRNA靶标的积聚。MiR390的过表达也导致配子体形成缓慢，增加了miR390触发的tasiRNA积累，并降低了tasiRNA靶标的水平。我们得出结论，由miR156、miR390和它们的靶标控制的基因调控网络控制着P.patens的发育变化。这个网络的大致轮廓和调控逻辑在开花植物中是保守的，尽管有一些修改。因此，部分保守的小RNA网络影响了具有完全不同身体计划的植物的发育时间。
microRNA156 (miR156) affects developmental timing in flowering plants. miR156 and its target relationships with members of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) gene family appear universally conserved in land plants, but the specific functions of miR156 outside of flowering plants are unknown. We find that miR156 promotes a developmental change from young filamentous protonemata to leafy gametophores in the moss Physcomitrella patens, opposite to its role as an inhibitor of development in flowering plants. P. patens miR156 also influences accumulation of trans-acting small interfering RNAs (tasiRNAs) dependent upon a second ancient microRNA, miR390. Both miR156 and miR390 directly target a single major tasiRNA primary transcript. Inhibition of miR156 function causes increased miR390-triggered tasiRNA accumulation and decreased accumulation of tasiRNA targets. Overexpression of miR390 also caused a slower formation of gametophores, elevated miR390-triggered tasiRNA accumulation, and reduced level of tasiRNA targets. We conclude that a gene regulatory network controlled by miR156, miR390, and their targets controls developmental change in P. patens. The broad outlines and regulatory logic of this network are conserved in flowering plants, albeit with some modifications. Partially conserved small RNA networks thus influence developmental timing in plants with radically different body plans.