Gut microbiota alterations reveal potential gut-brain axis changes in polycystic ovary syndrome
Gut microbiota alterations reveal potential gut-brain axis changes in polycystic ovary syndrome
复制标题
肠道微生物群的改变揭示了多囊卵巢综合征潜在的肠脑轴变化
DOI：
10.1007/s40618-020-01481-5
复制
发表时间：
2021-01-02
影响因子：
5.4
通讯作者：
Yang, D.
中科院分区：
文献类型：
--
作者：
Liang, Z.;Di, N.;Yang, D.
目的多囊卵巢综合征(PCOS)是一种常见的异质性内分泌疾病，伴有神经内分泌和代谢紊乱。肠道微生物区系被认为在代谢性疾病和神经递质的产生中起着关键作用。以前的研究已经报道了PCOS患者和动物模型肠道微生物区系的变化，然而，大多数文章没有考虑年龄或饮食对肠道微生物区系的影响。这项研究的目的是确定PCOS患者与年龄和BMI匹配的健康对照组妇女肠道微生物种类的差异。方法对20例PCOS患者(瘦肉型PCOS，PL组，10例；超重型PCOS组，PO组，n=10)和20例健康对照组(瘦肉型对照组，CL组，超重对照组，n=10)进行体检和膳食调查，并于月经周期第1~3天采血测定内分泌、代谢和炎症因子水平，非月经期采集粪便，测16S rDNA基因V4区序列，分析肠道微生物区系组成。评估了临床参数与不同种属之间的相关性。结果膳食分析显示多囊卵巢综合征患者膳食纤维、维生素D摄入量明显减少。我们的研究首次发现PCOS中产生伽马氨基丁酸(GABA)的菌种增加，包括Parabacteroids disasonis、Bacteroids Fragilis和E.colias，它们与血清黄体生成素水平和黄体生成素/卵泡刺激素比值显著正相关。结论PCOS中GABA产生菌的增多与血清黄体生成素水平及黄体生成素/卵泡刺激素比值呈正相关。总而言之，多囊卵巢综合征患者的肠道微生物失调与神经内分泌变化有关，揭示了多囊卵巢综合征患者潜在的肠道-脑轴。
Purpose Polycystic ovary syndrome (PCOS) is a common heterogeneous endocrine disorder companied with neuroendocrine and metabolic disorders. Gut microbiota has been implicated to play a key role in metabolic diseases and the production of neurotransmitters. Previous studies have reported the alterations in the gut microbiota of PCOS patients and animal models, however, most of the articles did not take the effect of age or diet on gut microbiota into account. The aim of this study was to identify the differential gut microbial species in PCOS patients compared with age and BMI-matched healthy control women. Methods We performed physical examinations and dietary survey in 20 women with PCOS (lean PCOS, PL, n = 10; overweight PCOS, PO, n = 10) and 20 healthy control women (lean control, CL, n = 10; overweight control, CO, n = 10), and collected the blood on the days 1-3 of the menstrual cycle for the measurement of endocrine and metabolic profiles, and inflammatory factors; and collected the feces in non-menstrual period to investigate the composition of gut microbiota by sequencing the V4 region of the 16S rDNA gene in fecal samples. The correlations between clinical parameters and the differential species were evaluated. Results Dietary analysis showed that the intake of dietary fiber, vitamin D were significantly decreased in PCOS. For the first time, our study found an increase of gamma-aminobutyric acid (GABA)-producing species in PCOS, including Parabacteroides distasonis, Bacteroides fragilis and Escherichia coli, which significantly positively correlated with serum LH levels and LH:FSH ratios. Conclusions GABA-producing bacteria that were increased in PCOS, including Parabacteroides distasonis, Bacteroides fragilis and Escherichia coli, showed positive relationship with serum LH levels and LH:FSH ratios. In conclusion, gut microbial dysbiosis in women with PCOS is associated with neuroendocrine changes, revealing a potential gut-brain axis in PCOS.