Human chondroitin 6-sulfotransferase: cloning, gene structure, and chromosomal localization.
Human chondroitin 6-sulfotransferase: cloning, gene structure, and chromosomal localization.
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DOI：
10.1016/s0925-4439(98)00028-3
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发表时间：
1998-07
期刊：
影响因子：
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通讯作者：
K. D. Mazany;T. Peng;C. Watson;I. Tabas;K. J. Williams
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文献类型：
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作者：
K. D. Mazany;T. Peng;C. Watson;I. Tabas;K. J. Williams
软骨素6-磺基转移酶（C6 ST）是软骨素6-硫酸盐生物合成的关键酶，软骨素6-硫酸盐是一种与软骨形成、肿瘤形成、动脉粥样硬化和其他过程有关的糖胺聚糖。C6 ST催化硫酸根从3′-磷酸腺苷5′-磷酸硫酸根转移到软骨素的N-乙酰半乳糖胺残基的碳6上。以已发表的禽类基因序列为基础，检索表达序列标签数据库（dbEST），以人软骨肉瘤和内皮细胞RNA为模板，通过5′-RACE技术获得部分cDNA序列。将我们的全长表达构建体稳定转染到CHO-K1细胞中导致细胞匀浆中C6 ST和硫酸角质素磺基转移酶（KSST）酶活性显著增加。预测的411个氨基酸序列的人C6 ST包含一个N-末端疏水结构域与膜插入一致，四个潜在的N-连接糖基化位点，几个蛋白磷酸化的共识序列，和一个RGD序列。人和鸡C6 ST cDNA共享51%的核苷酸同源性，40%的氨酰基同源性，和75%的氨酰基保守性。人C6 ST基因的结构已被阐明，并表现出一个内含子的编码区，该基因已被定位到人类11号染色体的辐射杂交板定位。
Chondroitin 6-sulfotransferase (C6ST) is the key enzyme in the biosynthesis of chondroitin 6-sulfate, a glycosaminoglycan implicated in chondrogenesis, neoplasia, atherosclerosis, and other processes. C6ST catalyzes the transfer of sulfate from 3′-phosphoadenosine 5′-phosphosulfate to carbon 6 of the N-acetylgalactosamine residues of chondroitin. Based on the previously published avian sequence, we searched the database of expressed sequence tags (dbEST) and obtained partial-length cDNAs that we completed by 5′-RACE using human chondrosarcoma and endothelial-cell RNA as template. Stable transfection of our full-length expression construct into CHO-K1 cells resulted in marked increases in C6ST and keratan sulfate sulfotransferase (KSST) enzymatic activities in cell homogenates. The predicted 411 amino acid sequence of human C6ST contains an N-terminal hydrophobic domain consistent with membrane insertion, four potential sites for N-linked glycosylation, several consensus sequences for protein phosphorylation, and one RGD sequence. The human and chick C6ST cDNA share 51% nucleotide identity, 40% amino acyl identity, and 75% amino acyl conservation. The human C6ST gene structure has been elucidated and exhibits an intron-less coding region, and the gene has been mapped to human chromosome 11 by radiation hybrid panel mapping.