DIFFERENTIAL-MODES OF ACTION OF FIBRONECTIN AND EPIDERMAL GROWTH-FACTOR ON RABBIT CORNEAL EPITHELIAL MIGRATION
DIFFERENTIAL-MODES OF ACTION OF FIBRONECTIN AND EPIDERMAL GROWTH-FACTOR ON RABBIT CORNEAL EPITHELIAL MIGRATION
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DOI：
10.1002/jcp.1041450323
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发表时间：
1990-12-01
影响因子：
5.6
通讯作者：
OTORI, T
中科院分区：
文献类型：
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作者：
NISHIDA, T;NAKAMURA, M;OTORI, T
为了明确纤维连接蛋白（FN）和表皮生长因子（EGF）在角膜创面愈合中的作用，我们将运动后的兔角膜块在含有其中一种药物的培养基中培养24小时，然后测量上皮层沿块侧迁移的路径长度。FN和EGF均以剂量依赖性方式显著刺激上皮细胞迁移。对EGF的反应至少有12小时的滞后时间，才能观察到刺激，但对fn刺激的迁移没有滞后时间。FN只有在持续存在的情况下才能发挥最大的作用。相比之下，暴露于EGF 6小时不会刺激上皮细胞迁移，但暴露于EGF 9小时的刺激效果与连续暴露24小时后观察到的效果相同。抗FN抗体抑制FN-和egf刺激的角膜上皮迁移。但抗egf抗体仅抑制egf刺激的迁移，对fn刺激的迁移无影响。这些结果表明，与FN不同，一旦上皮细胞识别其信号，EGF不需要存在。此外，EGF的刺激作用依赖于FN，而FN的刺激作用不依赖于EGF。因此，EGF对角膜上皮迁移的影响可能是由FN介导的。
In order to clarify the roles of fibronectin (FN) and epidermal growth factor (EGF) in corneal wound healing, we cultured blocks of exercised rabbit cornea for 24 hours in media containing one of these agents, then measured the length of the path of the epithelial layer that had migrated down the side of the block. Both FN and EGF stimulated epithelial migration significantly in a dose-dependent fashion. Responses to EGF involved a time lag of least 12 hours before stimulation could be observed, but there was no lag-time for FN-stimulated migration. FN was maximally effective only if it was continuously present. In contrast, exposure to EGF for 6 hours did not stimulate epithelial migration, but exposure for 9 hours resulted in the same stimulatory effects as were observed after 24 hours' continuous exposure. Anti-FN antibody inhibited the FN- and EGF-stimulated migration of corneal epithelium. But anti-EGF antibody inhibited only EGF-stimulated migration and had no effect on FN-stimulated migration. These results indicate that, unlike FN, EGF need not be present, once the epithelial cells have recognized its signal. Furthermore, the stimulatory effect of EGF depended on FN, while that of FN was independent of EGF. The effects of EGF on migration of corneal epithelium may, therefore, be mediated by FN.