MOUSE HEPATITIS-VIRUS STRAIN-A59 AND BLOCKING ANTIRECEPTOR MONOCLONAL-ANTIBODY BIND TO THE N-TERMINAL DOMAIN OF CELLULAR RECEPTOR
MOUSE HEPATITIS-VIRUS STRAIN-A59 AND BLOCKING ANTIRECEPTOR MONOCLONAL-ANTIBODY BIND TO THE N-TERMINAL DOMAIN OF CELLULAR RECEPTOR
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DOI：
10.1073/pnas.90.5.1716
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发表时间：
1993-03-01
影响因子：
11.1
通讯作者：
HOLMES, KV
中科院分区：
文献类型：
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作者：
DVEKSLER, GS;PENSIERO, MN;HOLMES, KV
小鼠肝炎病毒（MHV）菌株A59用作免疫球蛋白超家族中癌胚抗原家族的细胞受体成员。重组受体蛋白具有全部或部分免疫球蛋白结构域缺失的重组受体蛋白，以鉴定病毒和抗受体单克隆抗体CC1识别的受体糖蛋白区域，该抗体CC1阻断了鼠类细胞的感染。单克隆抗体CC1和MHV-A59病毒体仅与含有MHV受体的整个域的重组蛋白约束。为了确定哪种蛋白质可以用作功能病毒受体，用重组缺失克隆转染受体阴性仓鼠细胞，然后用MHV-A59病毒体挑战。受体活性需要第二个或第四个结构域以及跨膜和细胞质结构域的整个N末端结构域。缺乏第一个结构域或其C末端部分的重组蛋白不充当病毒受体。因此，与免疫球蛋白超家族中的其他病毒受体一样，包括CD4，脊髓灰质炎病毒受体和细胞间粘附分子1，MHV受体的N末端结构域也被病毒和阻塞单克隆抗体识别。
Mouse hepatitis virus (MHV) strain A59 uses as cellular receptors members of the carcinoembryonic antigen family in the immunoglobulin superfamily. Recombinant receptor proteins with deletions of whole or partial immunoglobulin domains were used to identify the regions of receptor glycoprotein recognized by virus and by antireceptor monoclonal antibody CC1, which blocks infection of murine cells. Monoclonal antibody CC1 and MHV-A59 virions bound only to recombinant proteins containing the entire first domain of MHV receptor. To determine which of the proteins could serve as functional virus receptors, receptor-negative hamster cells were transfected with recombinant deletion clones and then challenged with MHV-A59 virions. Receptor activity required the entire N-terminal domain with either the second or the fourth domain and the transmembrane and cytoplasmic domains. Recombinant proteins lacking the first domain or its C-terminal portion did not serve as viral receptors. Thus, like other virus receptors in the immunoglobulin superfamily, including CD4, poliovirus receptor, and intercellular adhesion molecule 1, the N-terminal domain of MHV receptor is recognized by the virus and the blocking monoclonal antibody.