Reproductive implication of D-aspartic acid in human pre-ovulatory follicular fluid
Reproductive implication of D-aspartic acid in human pre-ovulatory follicular fluid
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DOI：
10.1093/humrep/dem328
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发表时间：
2007-12-01
影响因子：
6.1
通讯作者：
Ronsini, S.
中科院分区：
文献类型：
--
作者：
D'Aniello, G.;Grieco, N.;Ronsini, S.
背景：在本研究中，我们报道了D-天冬氨酸(D-Asp)存在于人的卵泡液中，这种氨基酸的浓度可能与卵母细胞的质量有关。方法：采集20例体外受精患者的排卵前卵泡液。D-天门冬氨酸氧化酶法测定D-天冬氨酸浓度。结果：人卵泡液中D-天冬氨酸的平均浓度为14.98+/-4.51nmol/ml，其含量与患者年龄有显著差异。在22~34岁的年轻女性(A组)中，D-Asp的浓度为19.11±1.91nmol/NIJ，而在35~40岁的患者(B组)中，D-Asp浓度降至10.86±1.22nmol/ml(P<；0.01)。此外，D-天冬氨酸与卵母细胞质量有关，D-Asp卵泡浓度与优质中期II卵母细胞百分率及受精率之间存在一定的关系(P<；0.01)。结论：在人类卵泡液中，D-天冬氨酸在年轻女性中的浓度相对高于老年患者，并且D-天冬氨酸浓度与生育结局参数之间似乎存在关系。提示卵泡D-天冬氨酸浓度可作为体外受精患者卵母细胞质量的替代或附加生化指标。
BACKGROUND: In the present study, we report that D-aspartic acid (D-Asp) occurs in human ovarian follicular fluid and that a relationship may exist between the concentration of this amino acid and oocyte quality. METHODS: Samples of pre-ovulatory follicle fluid were obtained from 20 patients undergoing an IVF programme. The concentration Of D-Asp was measured by using specific high-performance liquid chromatography (HPLC) combined with a D-aspartate oxidase. RESULTS: D-Asp occurs in human follicular fluid at a mean concentration of 14.98 +/- 4.51 nmol/ml. A significant difference in the content of this amino acid in the follicular fluid in relation to patient's age exists. In younger women aged 22-34 years (group A), D-Asp was found at a concentration of 19.11 +/- 1.91 nmol/niJ, whereas in patients aged 35-40 years (group B), it decreased to 10.86 +/- 1.22 nmol/ml (P < 0.01). In addition, this amino acid was linked to oocyte quality; a relationship exists between D-Asp follicular concentration and the percentage of good quality metaphase II oocytes (P < 0.01), as well as the fertilization rate. CONCLUSIONS: In human follicular fluid, D-Asp is present at a relatively higher concentration in younger women than in older patients and there appears to be a relationship between the concentration Of D-Asp and fertility outcome parameters. These findings suggest that follicular D-Asp concentration may be considered as an alternative or additional biochemical marker for oocyte quality in patients undergoing IVF programmes.