Cell-cell coupling in CO2/H+-excited neurons in brainstem slices
Cell-cell coupling in CO2/H+-excited neurons in brainstem slices
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DOI：
10.1016/s0034-5687(01)00284-5
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发表时间：
2001-12-01
期刊：
影响因子：
--
通讯作者：
Putnam, RW
中科院分区：
文献类型：
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作者：
Dean, JB;Kinkade, EA;Putnam, RW
间接和直接的电和解剖学证据的假设，中央化学感受器神经元在背侧脑干（孤波，SC蓝斑，LC）耦合的间隙连接，主要在大鼠脑干切片，和用于研究脑切片间隙连接的方法，批评和审查。间隙连接允许细胞间通讯，这在电偶联（离子电流的细胞间流动）、代谢偶联（信号分子的细胞间流动）或两者中都很重要，最终影响呼吸性酸中毒期间SC和LC内的兴奋性。间隙连接也可以提供一种机制，用于在导致中枢呼吸化学敏感性增加或降低的条件下调节网络中的神经元活性。电耦合的间接测量表明，化学敏感神经元之间的连接电导对宽范围的细胞内pH（pH（i））相对不敏感，在35-37 ℃下，pH（i）约为7.49至约为6.71。相反，pH（i）的进一步降低，直到pH（i）接近6.67，消除了电耦合的间接测量。(C)2001 Elsevier Science B. V.保留所有权利。
The indirect and direct electrical and anatomical evidence for the hypothesis that central chemoreceptor neurons in the dorsal brainstem (solitary complex, SC; locus coeruleus, LC) are coupled by gap junctions, as reported primarily in rat brainstem slices, and the methods used to study gap junctions in brain slices, are critiqued and reviewed. Gap junctions allow intercellular communication that could be important in either electrical coupling (intercellular flow of ionic current), metabolic coupling (intercellular flow of signaling molecules), or both, ultimately influencing excitability within the SC and LC during respiratory acidosis. Gap junctions may also provide a mechanism for modulating neuronal activity in the network under conditions that lead to increased or decreased central respiratory chemosensitivity. Indirect measures of electrical coupling suggest that junctional conductance between chemosensitive neurons is relatively insensitive to a broad range of intracellular pH (pH(i)), ranging from pH(i) approximate to 7.49 to approximate to 6.71 at 35-37 degreesC. In contrast, further reductions in pH(i), down through pH(i) approximate to 6.67, abolish indirect measures of electrical coupling. (C) 2001 Elsevier Science B.V. All rights reserved.