Adrenomedullin regulates blood-brain barriers functions in vitro
Adrenomedullin regulates blood-brain barriers functions in vitro
复制标题
DOI：
10.1097/00001756-200112210-00055
复制
发表时间：
2001-12-21
期刊：
影响因子：
1.7
通讯作者：
Ueta, Y
中科院分区：
文献类型：
--
作者：
Kis, B;Deli, MA;Ueta, Y
肾上腺髓质素（Adrenomedullin，AM）是脑循环中重要的血管扩张剂，脑内皮细胞是AM的主要来源。本体外研究旨在确定AM诱导的血脑屏障（BBB）功能变化。所有管理增加，而AM反义寡核苷酸治疗降低跨内皮电阻。AM孵育降低了荧光素钠（mol. wt 376 Da），但不包括Evan蓝白蛋白（mol. wt 67 kDa），并且其还减弱了液相内吞作用。AM处理导致体外P-糖蛋白外排泵功能激活。我们的结果表明，AM作为一种自分泌介质在脑内皮细胞的BBB特性的调节中起着重要的作用。神经报告12：4139-4142。(C)2001年利平科特威廉姆斯&威尔金斯。
Adrenomedullin (AM) is an important vasodilator in cerebral circulation, and cerebral endothelial cells are a major source of AM. This in vitro study aimed to determine the AM-induced changes in blood-brain barrier (BBB) functions. All administration increased, whereas AM antisense oligonucleotide treatment decreased transendothelial electrical resistance. AM incubation decreased BBB permeability for sodium fluorescein (mol. wt 376 Da) but not for Evan's blue albumin (mol. wt 67 kDa), and it also attenuated fluid-phase endocytosis. AM treatment resulted in functional activation of P-glycoprotein efflux pump in vitro. Our results indicate that AM as an autocrine mediator plays an important role in the regulation of BBB properties of the cerebral endothelial cells. NeuroReport 12:4139-4142. (C) 2001 Lippincott Williams & Wilkins.