Bmc Developmental Biology Quantitative Evaluation and Selection of Reference Genes in Mouse Oocytes and Embryos Cultured in Vivo and in Vitro
Bmc Developmental Biology Quantitative Evaluation and Selection of Reference Genes in Mouse Oocytes and Embryos Cultured in Vivo and in Vitro
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DOI：
10.1007/s00284-016-1061-5
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发表时间：
2016-05
影响因子：
2.6
通讯作者：
S. Mamo;Á. B. Gál;S. Bodó;A. Dinnyés;Arpad Baji;Szilard
中科院分区：
文献类型：
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作者：
S. Mamo;Á. B. Gál;S. Bodó;A. Dinnyés;Arpad Baji;Szilard
现在已经明确的是，神经元功能障碍而不是结构损伤可能是狂犬病发生的原因。为了探索狂犬病病毒（RABV）和突触功能障碍的潜在机制，对小鼠海马突触体样本进行了定量蛋白质组分析。分离小鼠海马体的突触体样本，并通过蛋白质印迹和透射电子显微镜进行证实。通过 Nano-LC-MS/MS 定量检测突触体蛋白含量变化。蛋白质功能通过基因本体论（GO）和KEGG途径进行分类。 PSICQUIC 用于创建网络。应用MCODE算法获得子网络。在这些蛋白质变化中，相对于未感染（模拟）突触体，RABV 感染后 45 种蛋白质变化上调，14 种蛋白质变化下调。 28 种蛋白质是模拟治疗特有的，12 种蛋白质是 RABV 治疗特有的。与代谢和突触小泡相关的蛋白质的表达水平变化最大。此外，蛋白质-蛋白质相互作用（PPI）网络表明，与突触功能相关的几个关键生物过程可能受到 RABV 的调节，包括能量代谢、细胞骨架组织和突触传递。这些数据将有助于更好地了解狂犬病的神经元功能障碍，并为未来的研究奠定基础。
It is well established now that neuronal dysfunction rather than structural damage may be responsible for the development of rabies. In order to explore the underlying mechanisms in rabies virus (RABV) and synaptic dysfunctions, a quantitative proteome profiling was carried out on synaptosome samples from mice hippocampus. Synaptosome samples from mice hippocampus were isolated and confirmed by Western blot and transmission electron microscopy. Synaptosome protein content changes were quantitatively detected by Nano-LC–MS/MS. Protein functions were classified by the Gene Ontology (GO) and KEGG pathway. PSICQUIC was used to create a network. MCODE algorithm was applied to obtain subnetworks. Of these protein changes, 45 were upregulated and 14 were downregulated following RABV infection relative to non-infected (mock) synaptosomes. 28 proteins were unique to mock treatment and 12 were unique to RABV treatment. Proteins related to metabolism and synaptic vesicle showed the most changes in expression levels. Furthermore, protein–protein interaction (PPI) networks revealed that several key biological processes related to synaptic functions potentially were modulated by RABV, including energy metabolism, cytoskeleton organization, and synaptic transmission. These data will be useful for better understanding of neuronal dysfunction of rabies and provide the foundation for future research.