Mediation of alopecia areata by cooperation between CD4+ and CD8+ T lymphocytes -: Transfer to human scalp explants on Prkdcscid mice
Mediation of alopecia areata by cooperation between CD4+ and CD8+ T lymphocytes -: Transfer to human scalp explants on Prkdcscid mice
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DOI：
10.1001/archderm.138.7.916
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发表时间：
2002-07-01
影响因子：
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通讯作者：
Kalish, RS
中科院分区：
文献类型：
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作者：
Gilhar, A;Landau, M;Kalish, RS
目的：目的：探讨CD4（+）和CD8（+）T淋巴细胞在斑秃发病机制中的作用。注射前用磁珠分离CD4（+）和CD8（+）T细胞。设置：基于大学的皮肤病学实践参与者：11名全秃或严重斑秃患者。主要结果测量：毛发再生，毛发脱落和头皮外植体的免疫组化结果。干预：将头皮T细胞转移到Prkdc（scid）小鼠的自体病变头皮外植体。结果：注射未分离的T细胞和混合的CD4 ++ CD8 + T细胞导致明显的脱发（P
Objective: To determine the role of CD4(+) and CD8(+) T lymphocytes in the pathogenesis of alopecia areata.Design: Relapse of alopecia areata was induced in autologous human scalp grafts on Prkdc(scid) mice by injection of activated T lymphocytes derived from lesional skin. CD4(+) and CD8(+) T cells were separated by magnetic beads before injection.Setting: University-based dermatology practiceParticipants: Eleven patients with either alopecia totalis or severe alopecia areata.Main Outcome Measures: Hair regrowth, hair loss, and immunohistochemical findings of scalp explants.Intervention: Transfer of scalp T cells to autologous lesional scalp explants on Prkdc(scid) mice.Results: Injection of unseparated T cells and mixed CD4(+) plus CD8(+) T cells resulted in significant hair loss (P