Reactive Inkjet Printing of Functional Silk Stirrers for Enhanced Mixing and Sensing.
Reactive Inkjet Printing of Functional Silk Stirrers for Enhanced Mixing and Sensing.
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DOI：
10.1002/smll.201804213
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发表时间：
2018-12
期刊：
影响因子：
13.3
通讯作者：
Yu Zhang;D. Gregory;Yi Zhang;Patrick J. Smith;S. Ebbens;Xiubo Zhao
中科院分区：
文献类型：
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作者：
Yu Zhang;D. Gregory;Yi Zhang;Patrick J. Smith;S. Ebbens;Xiubo Zhao
搅拌小体积的溶液可以减少免疫测定读出时间，使细胞培养物均质化，并增加生物反应器中的酶反应性。然而，目前许多小规模搅拌方法需要外部致动，这可能是麻烦的。为了解决这个问题，在这里，反应性喷墨印刷被证明能够产生自主旋转的生物相容性基于丝的微搅拌器，可以增强流体混合。旋转运动是通过释放表面活性剂（小分子聚乙二醇）产生马兰戈尼效应，或通过催化动力气泡推进产生的。Marangoni驱动的设备不需要添加任何化学品到流体中作为“燃料”，而催化动力设备则通过分解溶液中的基质分子来提供动力。对Marangoni效应和酶动力搅拌器进行了比较。马兰戈尼效应驱动的搅拌器旋转高达600 rpm，比酶驱动的微搅拌器快75 - 100倍，然而酶驱动的搅拌器显示出更长的寿命。除了搅拌应用之外，运动产生机构对流体性质的敏感性允许旋转装置也用于感测应用，例如，用作水污染的运动传感器。
Stirring small volumes of solution can reduce immunoassay readout time, homogenize cell cultures, and increase enzyme reactivity in bioreactors. However, at present many small scale stirring methods require external actuation, which can be cumbersome. To address this, here, reactive inkjet printing is shown to be able to produce autonomously rotating biocompatible silk-based microstirrers that can enhance fluid mixing. Rotary motion is generated either by release of a surface active agent (small molecular polyethylene glycol) resulting in Marangoni effect, or by catalytically powered bubble propulsion. The Marangoni driven devices do not require any chemicals to be added to the fluid as the "fuel," while the catalytically powered devices are powered by decomposing substrate molecules in solution. A comparison of Marangoni effect and enzyme powered stirrers is made. Marangoni effect driven stirrers rotate up to 600 rpm, 75-100-fold faster than enzyme driven microstirrers, however enzyme powered stirrers show increased longevity. Further to stirring applications, the sensitivity of the motion generation mechanisms to fluid properties allows the rotating devices to also be exploited for sensing applications, for example, acting as motion sensors for water pollution.