Tentative identification of a resilin gene in Drosophila melanogaster
Tentative identification of a resilin gene in Drosophila melanogaster
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DOI：
10.1016/s0965-1748(01)00044-3
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发表时间：
2001-09-01
影响因子：
3.8
通讯作者：
Andersen, SO
中科院分区：
文献类型：
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作者：
Ardell, DH;Andersen, SO
在果蝇基因组中搜索与蝗虫(Schistocerca Gregaria)resilin三种胰蛋白酶多肽氨基酸序列相似的基因产物，得到两个阳性结果：基因产物CG15920和CG9036。在这两种概念翻译产品中都存在一个62个残基的区域，这与29个位置的resilin多肽相同。基因产物CG15920的氨基酸组成与多种昆虫的Resilins相似，其N端信号肽序列表明它是一种胞外蛋白。62个残基区与RR-2序列相似，这在昆虫固体角质层的许多基质蛋白中是常见的。CG 15920中62个残基两侧的N末端和C末端区域分别由15个残基序列的18个重复和13个残基序列的I I重复所主导。重复序列的结构预测，肽链将以不规则的、延长的β-螺旋方式折叠，类似于哺乳动物弹性蛋白和蜘蛛鞭毛状丝的结构，这两种材料与resilin一样，具有长期弹性。因此，我们认为CG15920基因产物是果蝇resilin的前体。(C)2001爱思唯尔科学有限公司。保留所有权利。
A search of the Drosophila genome for gene products with similarities to the amino acid sequences of three tryptic peptides from locust (Schistocerca gregaria) resilin gave two positive results: gene products CG15920 and CG9036. In both conceptual translation products a 62-residue region is present, which is identical to the resilin peptides in 29 positions. Gene product CG15920 has an amino acid composition closely resembling that of resilins from various insect species, and it has an N-terminal signal peptide sequence indicating that it is an extracellular protein. The 62-residue region shows similarity to the RR-2 sequence, which is common for a number of matrix proteins from insect solid cuticle. The N- and C-terminal regions flanking the 62-residue in CG 15920 are dominated by 18 repeats of a 15-residue sequence and I I repeats of a 13-residue sequence, respectively. The structures of the repeats predict that the peptide chain will fold in an irregular, extended beta-spiral, resembling the structures suggested for mammalian elastin and spider flagelliform silk, two materials which, like resilin, possess long-range elasticity. Accordingly, we suggest that gene product CG15920 is a Drosophila resilin precursor. (C) 2001 Elsevier Science Ltd. All rights reserved.