Susceptibility of common fibroblast cell lines to transmissible spongiform encephalopathy agents
Susceptibility of common fibroblast cell lines to transmissible spongiform encephalopathy agents
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DOI：
10.1086/381166
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发表时间：
2004-02-01
影响因子：
6.4
通讯作者：
Priola, SA
中科院分区：
文献类型：
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作者：
Vorberg, I;Raines, A;Priola, SA
由于使用动物产品作为培养基组分，认为组织培养细胞被传染性海绵状脑病（TSE）因子污染的风险较低，部分原因是仅报告了少数脑源性细胞系对TSE感染敏感。在本研究中，我们证明了常见的实验室成纤维细胞系NIH/3 T3和L929，表达低水平的细胞小鼠朊蛋白，容易感染小鼠适应性瘙痒症。我们的研究结果表明，细胞系对TSE感染的易感性不能根据其组织来源或其细胞朊病毒蛋白的表达水平来预测，并且他们表明，任何表达正常宿主朊病毒蛋白的细胞系都有可能支持TSE试剂的繁殖。因此，对于常规用于疫苗生产和其他医学应用的所有细胞系，可能需要检测细胞的TSE敏感性。
The risk of contamination of tissue culture cells with transmissible spongiform encephalopathy (TSE) agents as a result of the use of animal products as medium components has been considered to be low, in part, because only a few brain-derived cell lines have been reported to be susceptible to TSE infection. In the present study, we demonstrate that the common laboratory fibroblast cell lines NIH/3T3 and L929, which express low levels of cellular mouse prion protein, are susceptible to infection with mouse-adapted scrapie. Our results show that the susceptibility of a cell line to TSE infection cannot be predicted on the basis of its tissue origin or its level of expression of the cellular prion protein, and they suggest that any cell line expressing normal host prion protein could have the potential to support propagation of TSE agents. Thus, testing of cells for TSE susceptibility might be necessary for all cell lines that are routinely used in vaccine production and in other medical applications.