Molecular characterization and expression of the cholesteryl ester transfer protein gene in chickens
Molecular characterization and expression of the cholesteryl ester transfer protein gene in chickens
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DOI：
10.1016/j.cbpb.2007.05.003
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发表时间：
2007-10-01
影响因子：
2.2
通讯作者：
Akiba, Yukio
中科院分区：
文献类型：
--
作者：
Sato, Kan;Huchi, Aya;Akiba, Yukio
胆固醇酯转移蛋白（CETP）是一种催化血浆中极低密度脂蛋白和高密度脂蛋白之间胆固醇酯转移的蛋白质，从鸡肝中分离得到。当重组蛋白在HEK293细胞中过表达时，在培养基和细胞裂解物中观察到胆固醇酯转移活性。通过北方杂交分析，检测到鸡CETP mRNA在肝、脑、心、脾中的表达。鸡CETP mRNA表达和血浆CETP活性与营养状态的变化进行了检查，发现增加与人类相似的方式与胆固醇的饮食补充。肝CETP mRNA水平和血浆CETP活性显着低于成熟（即产蛋）母鸡比未成熟的雌性鸡，但不受年龄的雄性动物。雄性动物雌二醇给药后，观察到与雌性鸡中观察到的变化相似的变化。本研究首次报道了禽类CETP的分子特征，并证实CETP基因和活性的损伤可能受雌激素的调控，在蛋鸡产蛋过程中发挥重要作用，表明禽类和哺乳动物的脂质代谢存在种属特异性差异。(C)2007年爱思唯尔公司All rights reserved.
The cDNA for cholesteryl ester transfer protein (CETP), a protein that catalyzes cholesteryl ester transfer between very low density and high density lipoproteins in plasma, was isolated from chicken liver. When the recombinant protein was overexpressed in HEK293 cells, cholesteryl ester transfer activity was observed in media and cell lysates. By Northern blot analysis, chicken CETP mRNA expression was detected in liver, brain, heart, and spleen. Changes in chicken CETP mRNA expression and plasma CETP activity with nutritional state were examined and found to increase following dietary supplementation with cholesterol in a similar way as in humans. Both the hepatic CETP mRNA levels and plasma CETP activity were significantly lower in mature (i.e egg-laying) hens than in immature female chickens, but were unaffected by age in male animals. Similar changes to those observed in female chickens were observed upon estradiol administration of males. The present study is the first to report the molecular characterization of an avian CETP, and the impairments of CETP gene and activity, which might be regulated by estrogen, play an important role in egg production in laying hens, demonstrating species-specific differences in the lipid metabolism of avian and mammalian species. (C) 2007 Elsevier Inc. All rights reserved.