Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA
Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA
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由 DNA 制成的强效抗肿瘤免疫刺激生物相容性纳米水凝胶
DOI：
10.1186/s11671-019-3032-9
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发表时间：
2019-06-26
影响因子：
--
通讯作者：
Liu, Peifeng
中科院分区：
文献类型：
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作者：
Jiang, Jiana;Kong, Xianming;Liu, Peifeng
非甲基化CpG寡脱氧核苷酸（CpG oligodeoxynucleotides，CpG ODN）是一种有效的免疫刺激剂，通过诱导Th 1型抗原特异性T细胞应答，激活先天性和获得性免疫系统，但其在血清中的不稳定性严重影响其免疫刺激效率。在这里，我们构建了一种新型的免疫DNA纳米水凝胶组成的串联重复序列的CpG单位命名为CpG-MCA纳米水凝胶通过多引物链扩增。CpG-MCA纳米水凝胶被证明具有抗降解作用，并增加小鼠巨噬细胞样RAW264.7细胞的增殖和迁移。CpG-MCA纳米水凝胶可有效诱导肿瘤坏死因子-α和白细胞介素-6的高表达，并显著抑制U251细胞的增殖，有望成为一种有效的抗癌免疫刺激剂。
Unmethylated CpG oligodeoxynucleotides are potent immunostimulatory motifs in activating both innate and acquired immune system by inducing Th1 type antigen-specific T cell responses, but their instability in serum greatly influences their immunostimulant efficiency. Here, we constructed a novel immuno-DNA nanohydrogels consisting of tandem repeat sequences of CpG units named CpG-MCA nanohydrogels through multi-primed chain amplification. CpG-MCA nanohydrogels were proved to resist degradation and increase the proliferation and migration of murine macrophage-like RAW264.7 cells. Furthermore, CpG-MCA nanohydrogels effectively induced high expression of tumor necrosis factor-α and interleukin-6, and remarkably inhibited the proliferation of U251 cells, suggesting that CpG-MCA nanohydrogels are expected to be employed as the potent anti-cancer immunostimulant.