Aurora controls sister kinetochoremono-orientation and homolog bi-orientation in meiosis-I
Aurora controls sister kinetochoremono-orientation and homolog bi-orientation in meiosis-I
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DOI：
10.1038/sj.emboj.7601880
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发表时间：
2007-10-31
期刊：
影响因子：
11.4
通讯作者：
Watanabe, Yoshinori
中科院分区：
文献类型：
--
作者：
Hauf, Silke;Biswas, Ashapurno;Watanabe, Yoshinori
Aurora-B 激酶是有丝分裂染色体分离的重要调节因子，它们是姐妹染色单体忠实双向定向所必需的。与有丝分裂相反，姐妹染色单体在减数分裂 I 中必须朝向相同的纺锤体极，而同源染色体是双向的。我们发现裂殖酵母 Aurora 激酶 Ark1 对于有丝分裂中姐妹染色单体和减数分裂 I 中同源染色体的忠实双向定位是必需的。出乎意料的是，Ark1对于减数分裂-I中姐妹染色单体的忠实单方向也是必需的，尽管依赖于Moa1和Rec8的规范单方向途径似乎完好无损。我们的数据表明，Ark1 可以防止中期 I 期间统一的姐妹动粒与两个纺锤体极的裂隙附着，从而防止在后期 I 期间被撕裂，揭示了一种促进单极附着的新机制。此外，我们的结果为之前神秘的观察结果提供了解释，即裂殖酵母 Shugoshin Sgo2 有助于将 Aurora 加载到着丝粒，其调节因子 Bub1 对于减数分裂 1I 中姐妹染色单体的单向是必需的。
Aurora-B kinases are important regulators of mitotic chromosome segregation, where they are required for the faithful bi-orientation of sister chromatids. In contrast to mitosis, sister chromatids have to be oriented toward the same spindle pole in meiosis-I, while homologous chromosomes are bi-oriented. We find that the fission yeast Aurora kinase Ark1 is required for the faithful bi-orientation of sister chromatids in mitosis and of homologous chromosomes in meiosis-I. Unexpectedly, Ark1 is also necessary for the faithful mono-orientation of sister chromatids in meiosis-I, even though the canonical mono-orientation pathway, which depends on Moa1 and Rec8, seems intact. Our data suggest that Ark1 prevents unified sister kinetochores during metaphase-I from merotelic attachment to both spindle poles and thus from being torn apart during anaphase-I, revealing a novel mechanism promoting monopolar attachment. Furthermore, our results provide an explanation for the previously enigmatic observation that fission yeast Shugoshin Sgo2, which assists in loading Aurora to centromeres, and its regulator Bub1 are required for the mono-orientation of sister chromatids in meiosis-1I.