Twisting of neocortical progenitor cells underlies a spring-like mechanism for daughter-cell migration
Twisting of neocortical progenitor cells underlies a spring-like mechanism for daughter-cell migration
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DOI：
10.1016/j.cub.2006.11.023
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发表时间：
2007-01-23
期刊：
影响因子：
9.2
通讯作者：
Ogawa, Masaharu
中科院分区：
文献类型：
--
作者：
Miyata, Takaki;Ogawa, Masaharu
在胚胎发生过程中，哺乳动物新皮层壁通过祖细胞增殖[1-4]和子细胞向软膜表面迁移[5-8]而广泛增厚。进行了延时成像和功能实验，以便研究这些过程中可能涉及的机械力。当双极细胞连接的小鼠大脑壁的心室和软膜表面失去其心室附着，他们进行向外部区域，其中包含分化的神经元体易位。这些过渡单极细胞的软脑膜过程表现出卷曲或发夹环形态，表明软脑膜过程的扭曲和拉伸建立了一个弹簧样的机制，在心室脱离时将子细胞推向软脑膜表面。该模型得到了显微手术横断软脑膜过程中观察到的形态学变化的支持。药理学实验进一步揭示了中间丝参与软脑膜过程的扭曲。这些结果揭示了细胞迁移的新机制，并为详细研究机械力在3D大脑发育中的作用提供了有价值的工具。
The mammalian neocortical wall thickens extensively during embryogenesis via proliferation of progenitor cells [1-4] and migration of daughter cells toward the pial surface [5-8]. Time-lapse imaging and functional experiments were carried out so that the possible involvement of mechanical forces in these processes could be examined. When bipolar cells connecting the ventricular and pial surfaces of the mouse cerebral wall lose their ventricular attachment, they undergo somal translocation toward the outer zones, which contain differentiated neurons. The pial process of these transitioning unipolar cells exhibits a coiled or hairpin-loop morphology, suggesting that twisting and stretching of the pial process establishes a spring-like mechanism that propels the daughter cell toward the pial surface upon ventricular detachment. This model is supported by morphological changes observed in microsurgically transected pial processes. Pharmacological experiments further reveal the involvement of intermediate filaments in twisting of pial processes. These results uncover a novel mechanism for cellular migration and provide valuable tools for the detailed study of the role of mechanical forces in 3D brain development.