Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice.
Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice.
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超声靶向微泡破坏介导的单纯疱疹病毒胸苷激酶基因治疗小鼠肝癌
DOI：
10.1186/1756-9966-29-170
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发表时间：
2010-12-23
期刊：
影响因子：
--
通讯作者：
Liu C
中科院分区：
文献类型：
--
作者：
Zhou S;Li S;Liu Z;Tang Y;Wang Z;Gong J;Liu C
ObjectiveThe purpose of the study was to explore the anti-tumor effect of ultrasound -targeted microbubble destruction mediated herpes simplex virus thymidine kinase (HSV-TK) suicide gene system on mice hepatoma.MethodsForty mice were randomly divided into four groups after the models of subcutaneous transplantation tumors were estabilished: (1) PBS; (2) HSV-TK (3) HSV-TK+ ultrasound (HSV-TK+US); (4) HSV-TK+ultrasound+microbubbles (HSV-TK+US+MB). The TK protein expression in liver cancer was detected by western-blot. Applying TUNEL staining detected tumor cell apoptosis. At last, the inhibition rates and survival time of the animals were compared among all groups.ResultsThe TK protein expression of HSV-TK+MB+US group in tumor-bearing mice tissues were significantly higher than those in other groups. The tumor inhibitory effect of ultrasound-targeted microbubble destruction mediated HSV-TK on mice transplantable tumor was significantly higher than those in other groups (p < 0.05), and can significantly improve the survival time of tumor-bearing mice.ConclusionUltrasound-targeted microbubble destruction can effectively transfect HSV-TK gene into target tissues and play a significant inhibition effect on tumors, which provides a new strategy for gene therapy in liver cancer.
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影响因子：
2.9
作者：
Aoi, Atsuko;Watanabe, Yukiko;Kodama, Tetsuya
通讯作者：
Kodama, Tetsuya
影响因子：
2.1
作者：
Wang, ZG;Ling, ZY;Pu, SY
通讯作者：
Pu, SY
影响因子：
12.4
作者：
Endoh, M;Koibuchi, N;Kaneda, Y
通讯作者：
Kaneda, Y
DOI：
10.1109/58.896136
发表时间：
2001-01-01
影响因子：
3.6
作者：
Chomas, JE;Dayton, P;Ferrara, KW
通讯作者：
Ferrara, KW
影响因子：
2.9
作者：
Feril, LB;Kondo, T;Nakamura, S
通讯作者：
Nakamura, S