Detection of foodborne pathogens using surface plasmon resonance biosensors
Detection of foodborne pathogens using surface plasmon resonance biosensors
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DOI：
10.1016/s0925-4005(00)00717-6
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发表时间：
2001-04-15
影响因子：
8.4
通讯作者：
Rosicky, J
中科院分区：
文献类型：
--
作者：
Koubová, V;Brynda, E;Rosicky, J
使用基于表面等离子体激元棱镜激发和光谱询问的表面等离子体共振 (SPR) 传感器实时检测肠炎沙门氏菌和单核细胞增生李斯特氏菌。针对病原体的相应抗体作为共价交联双层固定在金传感器表面上或共价结合在交联白蛋白层上。传感器可以检测浓度低至 10(6) 个细胞/ml 的沙门氏菌和李斯特菌。传感器的灵敏度与使用相同抗体的标准 ELISA 相当。 (C) 2001 Elsevier Science B.V. 保留所有权利。
Salmonella enteritidis and Listeria monocytogenes were detected in real time using a surface plasmon resonance (SPR) sensor based on prism excitation of surface plasmons and spectral interrogation. The respective antibodies against the pathogens were immobilized on the gold sensor surface as a covalently crosslinked double-layer or covalently bound on a cross-linked albumin layer. Salmonella and Listeria could be detected by the sensor at concentrations down to 10(6) cell/ml. The sensor sensitivity was comparable with that of standard ELISA in which the same antibodies were used. (C) 2001 Elsevier Science B.V. All rights reserved.