Dynamic cell behavior on synthetic hydrogels with different charge densities
Dynamic cell behavior on synthetic hydrogels with different charge densities
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DOI：
10.1039/b818586g
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发表时间：
2009-01-01
期刊：
影响因子：
3.4
通讯作者：
Gong, Jian Ping
中科院分区：
文献类型：
--
作者：
Chen, Yong Mei;Ogawa, Rie;Gong, Jian Ping
制备了一系列具有-8.8--20.5mV范围内的ζ电位（ζ）和约20.5mV的恒定杨氏模量E）的聚（NaSS-共-DMAAm）水凝胶。200 kPa的压力下研究了水凝胶的电荷密度对动态电池行为的影响。通过调节用于凝胶化的单体混合物[ NaSS和N，N-二甲基丙烯酰胺DMAAm]中所含的带负电荷的对苯乙烯磺酸钠（NaSS）的摩尔摩擦来调节ζ。观察到控制细胞行为的临界zeta电位zeta（crit）= -14.0 mV。当zeta > zeta（crit）时，细胞表现出小的铺展面积、快的迁移速度和大的迁移距离，同时存在肌动蛋白纤维的缺陷和不太突出的局灶性粘连。相反，当zeta zeta（临界），虽然无法增殖。细胞行为与水凝胶表面吸附的纤连蛋白密切相关。
A series of polyNaSS-co-DMAAm) hydrogels with zeta potentials (zeta) in the range -8.8--20.5 mV and a constant Young's modulus E) of ca. 200 kPa were designed for studying the effect of the charge density of hydrogels on dynamic cell behavior. The zeta was adjusted by tuning the molar friction of negatively charged sodium p-styrene sulfonate NaSS) contained in the monomer mixture [ NaSS and N, N-dimethyl acrylamide DMAAm)] for gelation. A critical zeta potential, which controls cell behavior, zeta(crit) = -14.0 mV, was observed. When zeta > zeta(crit), the cells exhibited a small spreading area, fast migration velocity, and a large migration distance, with concurrent deficiency in actin fibers and less prominent focal adhesions. Conversely, when zeta zeta(crit), although were unable to proliferate. The cell behavior is well correlated with the adsorbed fibronectin on the hydrogel surfaces.