A simple and rapid optical biosensor for detection of aflatoxin B1 based on competitive dispersion of gold nanorods
A simple and rapid optical biosensor for detection of aflatoxin B1 based on competitive dispersion of gold nanorods
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DOI：
10.1016/j.bios.2013.03.048
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发表时间：
2013-09-15
影响因子：
12.6
通讯作者：
Ying, Yibin
中科院分区：
文献类型：
--
作者：
Xu, Xia;Liu, Xiangjiang;Ying, Yibin
这份报告说明了一个有前途的一步和无标记的光学生物传感器，用于测定黄曲霉毒素B1（AFB 1），这是最常见的食品和高度危险，即使在非常低的浓度。在这项研究中，金纳米棒（GNRs）被用作传感平台，它在高离子强度条件下显示出高稳定性，而无需添加任何稳定剂。GNR-AFB 1-BSA（牛血清白蛋白）结合物与游离抗体混合后发生聚集，导致吸收强度发生显著变化。同时，样品中AFB 1分子的存在导致纳米棒分散，这是与抗体竞争免疫反应的结果。通过利用GNRs的竞争性分散，所开发的方法可以有效地减少由不期望的聚集引起的错误结果，这是球形金纳米颗粒的一个大问题。紫外-可见光谱的吸收强度作为传感指标，动态光散射（DLS）测量作为另一种传感工具。所设计的生物传感系统可以在0.5至20 ng/mL（-1）的线性范围内检测AFB 1，相关系数为0.99。检测限为0.16 ng/mL，表明吸光度结果具有良好的灵敏度。在真实的花生样品中的加标回收率为94.2%~ 117.3%。因此，纳米生物传感器被证明是敏感的，选择性，和简单的，提供了一个可行的替代快速筛选农产品和食品中的毒素。(C)2013爱思唯尔有限公司版权所有。
This report illustrates a promising one-step and label-free optical biosensor for determination of aflatoxin B1 (AFB1) that is most commonly found in foods and highly dangerous even at very low concentrations. In this research, gold nanorods (GNRs) were employed as a sensing platform, which showed high stability under high ionic strength conditions without addition of any stabilizing agent. GNR-AFB1-BSA (bovine serum albumin) conjugates aggregated after mixing with free antibodies, resulting in significant changes in absorption intensity. At the same time the existence of AFB1 molecules in samples caused dispersion of nanorods, as a result of competitive immune-reaction with antibodies. By taking advantages of the competitive dispersion of GNRs, the developed method could effectively reduce false results caused by undesirable aggregation, which is a big problem for spherical gold nanoparticles. Absorption intensity of UV-vis spectra served as the sensing indicator, with dynamic light scattering (DLS) measurement as another sensing tool. The designed biosensing system could detect AFB1 in a linear range from 0.5 to 20 ng mL(-1), with a good correlation coefficient of 0.99. And the limit of detection CLOD) was 0.16 ng mL(-1), indicating an excellent sensitivity with absorbance result. The recoveries of the spiked AFB1 in real peanut samples ranged from 94.2% to 117.3%. Therefore the proposed nano-biosensor was demonstrated to be sensitive, selective, and simple, providing a viable alternative for rapid screening of toxins in agriculture products and foods. (C) 2013 Elsevier B.V. All rights reserved.