Novel MHC class I-related molecule MR1 affects MHC class I expression in 293T cells
Novel MHC class I-related molecule MR1 affects MHC class I expression in 293T cells
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DOI：
10.1016/j.bbrc.2007.11.156
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发表时间：
2008-02-08
影响因子：
3.1
通讯作者：
Aldemir, Hatice
中科院分区：
文献类型：
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作者：
Aldemir, Hatice
与β（2）-微球蛋白的缔合和结合配体是抗原呈递分子在细胞表面表达的必要条件。MHCI类相关蛋白MR 1被认为具有抗原提呈功能，但其生理配体仍有待确定。在本研究中，通过表征人MR 1转染子的亚细胞行为，我们已经显示了其差异动员。我们的研究结果表明，在MHC I类缺陷B细胞系中，MR 1与β（2）-微球蛋白优先相关。此外，我们已经证明了经典的MHC I类分子在人MR 1转染的293 T细胞中的表达减少，表明MR 1和经典的MHC I类分子之间可能存在相互作用。(C)2007年爱思唯尔公司All rights reserved.
Association with beta(2)-microglobulin and binding a ligand are necessary conditions for cell surface expression of the antigen presenting molecules. MHC class I-related protein, MR1, is suggested to have an antigen presentation function, nevertheless the physiological ligand(s) is (are) still to be determined. In the present study, by characterising the subcellular deportment of human MR1 transfectants, we have shown its differential mobilisation. Our results demonstrated a preferential association of MR1 with beta(2)-microglobulin in MHC class I-deficient B cell lines. Furthermore, we have evidenced diminished expression of classical MHC class I molecules in human MR1-transfected 293T cells, showing a possible interaction between MR1 and classical MHC class I molecules. (C) 2007 Elsevier Inc. All rights reserved.