Pycnogenol® and vitamin E inhibit ethanol-induced apoptosis in rat cerebellar granule cells
Pycnogenol® and vitamin E inhibit ethanol-induced apoptosis in rat cerebellar granule cells
复制标题
DOI：
10.1002/neu.10311
复制
发表时间：
2004-06-01
期刊：
影响因子：
--
通讯作者：
Heaton, MB
中科院分区：
文献类型：
--
作者：
Siler-Marsiglio, KI;Shaw, G;Heaton, MB
碧萝香酚(R)(PYC)是一种从法国海松(Pinus Maritima)树皮中提取的生物黄酮类专利化合物，清除自由基，促进细胞健康。PYC对神经元乙醇毒性的保护作用尚未见报道。本研究表明，在出生后第9天(P9)的大鼠小脑颗粒细胞中，抗氧化剂维生素E(VE)和PYC(1)可剂量依赖性地阻断400、800和1600 mg/dL乙醇暴露后的细胞死亡；(2)在同一模型系统中抑制乙醇诱导的caspase-3的激活；以及(3)通过磷脂酰丝氨酸转位到细胞表面来减少神经细胞膜的破坏。这些结果表明，PYC和VE都有可能作为治疗剂，拮抗乙醇诱导的神经细胞死亡。(C)2004年威利期刊公司。
Pycnogenol(R) (PYC), a patented combination of bioflavonoids extracted from the bark of French maritime pine (Pinus maritima), scavenges free radicals and promotes cellular health. The protective capacity of PYC against ethanol toxicity of neurons has not previously been explored. The present study demonstrates that in postnatal day 9 (P9) rat cerebellar granule cells the antioxidants vitamin E (VE) and PYC (1) dose dependently block cell death following 400, 800, and 1600 mg/dL ethanol exposure (2) inhibit the ethanol-induced activation of caspase-3 in the same model system; and (3) reduce neuronal membrane disruption as assayed by phosphatidylserine translocation to the cell surface. These results suggest that both PYC and VE have the potential to act as therapeutic agents, antagonizing the induction of neuronal cell death by ethanol exposure. (C) 2004 Wiley Periodicals, Inc.