Ribonucleotide reductase in the archaeon Pyrococcus furiosus: a critical enzyme in the evolution of DNA genomes?
Ribonucleotide reductase in the archaeon Pyrococcus furiosus: a critical enzyme in the evolution of DNA genomes?
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古细菌激烈热球菌中的核糖核苷酸还原酶：DNA 基因组进化中的关键酶？
DOI：
10.1073/pnas.94.2.475
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发表时间：
1997
影响因子：
11.1
通讯作者：
Marc Fontecave
中科院分区：
文献类型：
--
作者：
Joan Riera;Frank T. Robb;Robert B. Weiss;Marc Fontecave
核糖核苷酸还原酶（RNR），负责脱氧核糖核苷酸合成的酶，已被分离出激烈的热球菌，一个深分支超嗜热，严格厌氧古菌。它的基因已被克隆，测序，并显示有两个插入编码内含肽。纯化的酶绝对需要腺苷钴胺素的活性，这一特性将其定义为II类（腺苷钴胺素依赖性）原核RNR的成员。另一方面，古生菌RNR具有显着的氨基酸序列同源性与I类（需氧非血红素铁依赖性）和III类（厌氧铁硫依赖性）RNR存在于真核生物和细菌，分别。有人提出，这种酶可能是最接近的原始RNR，这使得关键的“RNA世界”到“DNA世界”的过渡可能的亲戚，而不同类别的当今RNR是分歧进化的产物。
Ribonucleotide reductase (RNR), the enzyme responsible for deoxyribonucleotide synthesis, has been isolated from Pyrococcus furiosus, a deeply branching hyperthermophilic, strictly anaerobic archaeon. Its gene has been cloned, sequenced, and shown to harbor two insertions encoding inteins. The purified enzyme absolutely requires adenosylcobalamin for activity, a trait that defines it as a member of class II (adenosyl-cobalamin-dependent) prokaryotic RNRs. On the other hand, the archaeal RNR has significant amino acid sequence homology with class I (aerobic non-heme iron-dependent) and class III (anaerobic iron-sulfur-dependent) RNRs present in eukaryotes and bacteria, respectively. It is proposed that this enzyme may be the closest possible relative of the original RNR, which allowed the key "RNA world" to "DNA world" transition, and that the different classes of present-day RNRs are the products of divergent evolution.
影响因子：
56.9
作者：
BOLLINGER, JM;EDMONDSON, DE;STUBBE, J
通讯作者：
STUBBE, J