Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA.
Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA.
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DOI：
10.3390/pathogens3020404
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发表时间：
2014-05-06
期刊：
影响因子：
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通讯作者：
Venkitanarayanan K
中科院分区：
文献类型：
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作者：
Amalaradjou MA;Venkitanarayanan K
每年有数以百万计的留置装置植入患者体内，葡萄球菌（S。金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和耐万古霉素金黄色葡萄球菌。金黄色葡萄球菌（VRSA））是与这些装置相关的大多数感染的原因，从而导致治疗失败。一旦建立，葡萄球菌生物膜变得对抗菌剂治疗和宿主反应具有抗性，从而作为复发性感染的病原体。本研究探讨了盐酸奥替尼啶（OH）抑制生物膜合成和灭活完全形成的葡萄球菌生物膜在不同的基质中的血清蛋白的存在和不存在的效力。接种S.在37 °C下用OH（0、0.5、1、2mM）处理金黄色葡萄球菌、MRSA或VRSA以防止生物膜形成。此外，研究了OH（0、2.5、5、10 mM）对聚苯乙烯板、不锈钢试样和导尿管上完全形成的葡萄球菌生物膜的生物膜效应。OH能有效地快速灭活S.金黄色葡萄球菌、MRSA和VRSA在聚苯乙烯板、不锈钢试样和导尿管上，在存在和不存在血清蛋白的情况下。使用2和10 mM OH完全灭活S.金黄色葡萄球菌增殖细胞和生物膜（>6.0 log减少）。此外，共聚焦成像揭示了与对照中的完整活生物膜相比，OH处理的样品中存在死细胞和生物膜结构的损失。结果表明，OH可以作为一种有效的抗菌剂来控制S.金黄色葡萄球菌，MRSA和VRSA的适当的医院表面和留置装置。
Millions of indwelling devices are implanted in patients every year, and staphylococci (S. aureus, MRSA and vancomycin-resistant S. aureus (VRSA)) are responsible for a majority of infections associated with these devices, thereby leading to treatment failures. Once established, staphylococcal biofilms become resistant to antimicrobial treatment and host response, thereby serving as the etiological agent for recurrent infections. This study investigated the efficacy of octenidine hydrochloride (OH) for inhibiting biofilm synthesis and inactivating fully-formed staphylococcal biofilm on different matrices in the presence and absence of serum protein. Polystyrene plates and stainless steel coupons inoculated with S. aureus, MRSA or VRSA were treated with OH (zero, 0.5, one, 2 mM) at 37 °C for the prevention of biofilm formation. Additionally, the antibiofilm effect of OH (zero, 2.5, five, 10 mM) on fully-formed staphylococcal biofilms on polystyrene plates, stainless steel coupons and urinary catheters was investigated. OH was effective in rapidly inactivating planktonic and biofilm cells of S. aureus, MRSA and VRSA on polystyrene plates, stainless steel coupons and urinary catheters in the presence and absence of serum proteins. The use of two and 10 mM OH completely inactivated S. aureus planktonic cells and biofilm (>6.0 log reduction) on all matrices tested immediately upon exposure. Further, confocal imaging revealed the presence of dead cells and loss in biofilm architecture in the OH-treated samples when compared to intact live biofilm in the control. Results suggest that OH could be applied as an effective antimicrobial to control biofilms of S. aureus, MRSA and VRSA on appropriate hospital surfaces and indwelling devices.