The human relaxin receptor (LGR7): Expression in the fetal membranes and placenta
The human relaxin receptor (LGR7): Expression in the fetal membranes and placenta
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DOI：
10.1016/j.placenta.2005.07.011
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发表时间：
2006-06-01
期刊：
影响因子：
3.8
通讯作者：
Bryant-Greenwood, G. D.
中科院分区：
文献类型：
--
作者：
Lowndes, K.;Amano, A.;Bryant-Greenwood, G. D.
松弛素受体最近被描述为富含亮氨酸的重复G蛋白偶联受体，并被命名为LGR 7。一种密切相关的受体LGR 8由一些细胞共表达。本研究通过RT-PCR方法检测了这些受体基因在人胎膜和胎盘中的表达，并通过免疫定位方法检测了LGR 7蛋白的表达。结果表明，LGR 7在胎膜中表达较好，在蜕膜中的表达显著高于羊膜（p < 0.05）。另一方面，胎盘中表达水平相对较低。LGR 7的主要剪接变体在胎盘或胎膜中均检测不到。在任一组织中LGR 8的表达也低于可检测水平。LGR 7的免疫染色用抗其胞内域和胞外域的抗血清进行，以寻找溶解的胞外域的证据。然而，两种抗血清获得了相似的染色模式，主要染色在羊膜上皮细胞、绒毛膜细胞滋养层细胞和蜕膜中。收集早产、分娩前后或早产胎膜早破（PPROM）和分娩后的全层胎膜。此外，在足月时，在自然分娩之前和之后的膜用于分析LGR 7基因表达。有显着更大的LGR 7表达（p = 0.01）在早产期相比，术语，表明松弛素在这个时候的潜在重要作用。在早产和足月分娩后，LGR 7基因表达略有下降，但没有达到显著性。免疫染色模式显示患者间变异性低于基因表达，临产和分娩后LGR 7染色更强烈。
The relaxin receptor has been recently described as a leucine-rich repeat G-protein coupled receptor and designated as LGR7. A closely related receptor, LGR8, is co-expressed by some cells. This study explored the expression of the genes for these receptors in the human fetal membranes and placenta by RT-PCR and the LGR7 protein by immunolocalization. The results showed that LGR7 was well expressed in the fetal membranes, with significantly more in the decidua (p < 0.05) than in the amnion. On the other hand, relatively low levels were expressed in the placenta. The major splice variant of LGR7 was undetectable in either the placenta or fetal membranes. Expression of LGR8 was also below the level of detectability in either tissue. Immunostaining for LGR7 was conducted with antisera to both its endomain and ectodomain, in order to seek evidence for a solubilized ectodomain. However, similar staining patterns were obtained with both antisera, with predominant staining in the cells of the amniotic epithelium, chorionic cytotrophoblast and decidua. Full-thickness fetal membranes from preterm deliveries, before and after labor or after preterm premature rupture of the membrane (PPROM) and labor were collected. In addition, membranes at term, both before and after spontaneous labor were used for analysis of LGR7 gene expression. There was significantly greater LGR7 expressed (p = 0.01) in the preterm period compared to term, indicating a potentially important role for relaxin at this time. There was a marginal decline in LGR7 gene expression after labor and delivery both at preterm and term, which did not reach significance. Immunostaining patterns showed less inter-patient variability than did gene expression, with more intense staining for LGR7 after labor and delivery.