Increases in fragmented glial fibrillary acidic protein levels in the spinal cords of patients with amyotrophic lateral sclerosis
Increases in fragmented glial fibrillary acidic protein levels in the spinal cords of patients with amyotrophic lateral sclerosis
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DOI：
10.1023/a:1022476724381
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发表时间：
1998-02-01
影响因子：
4.4
通讯作者：
Nagata, Y
中科院分区：
文献类型：
--
作者：
Fujita, K;Kato, T;Nagata, Y
采用一维聚丙烯酰胺凝胶电泳法，对肌萎缩侧索硬化症(ALS)患者脊髓提取的蛋白质组分进行了分析。肌萎缩侧索硬化症患者脊髓中考马斯亮蓝染色明显强于非肌萎缩侧索硬化症患者脊髓，其相对分子质量为35-55 kDa。蛋白序列分析显示，胶质纤维酸性蛋白(GFAP)的50和45 kDa条带显著减少，36和37 kDa条带明显增加，分别代表GFAP N末端缺失59和40个残基的片段。免疫组织化学检查发现萎缩的脊髓腹角内GFAP染色的星形胶质细胞增多，运动神经元大量变性。这些结果将为星形胶质细胞在ALS的病理生理学和/或发病机制中的可能作用提供新的见解。
Using one-dimensional polyacrylamide gel electrophoresis, we analyzed protein fractions extracted from the spinal cords of patients with amyotrophic lateral sclerosis (ALS). Several protein bands with molecular weights of 35-55 kDa were stained with Coomassie brilliant blue much more intensely in the ALS than in the non-ALS spinal cord. Glial fibrillary acidic protein (GFAP) immunoreactivity showed a significant decrease of 50 and 45 kDa band and increase in fragmented 36 and 37 kDa bands, which represented GFAP fragments devoid of 59 and 40 residues from the N-terminal, respectively, as determined by protein sequence analysis. Immunohistochemical examination of ALS spinal cord transections demonstrated increased GFAP-stained astrocytes in the shrunken ventral horn with massive degeneration of motoneurons. These results will provide new insight into the possible role of astrocytes in the pathophysiology and/or pathogenesis of ALS.