Excitability and contractility of skeletal muscle engineered from primary cultures and cell lines
Excitability and contractility of skeletal muscle engineered from primary cultures and cell lines
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DOI：
10.1152/ajpcell.2001.280.2.c288
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发表时间：
2001-02-01
影响因子：
5.5
通讯作者：
Faulkner, JA
中科院分区：
文献类型：
--
作者：
Dennis, RG;Kosnik, PE;Faulkner, JA
本研究的目的是比较的兴奋性和收缩性的三维骨骼肌结构，称为肌样细胞，工程从C2 C1/2成肌细胞和10 T 1/2成纤维细胞系，原代肌肉培养物从成年C3 H小鼠，新生儿和成年Sprague-Dawley大鼠。肌样细胞长12 mm，直径为0.1-1 mm，可通过横向电刺激兴奋，并收缩以产生力。在类似于30天的培养后，测量肌样横截面积、基强度、时值、静息基线力、抽搐力、达到峰值张力的时间、一半松弛时间和峰值等长力。通过将峰值等长力除以横截面积来计算比力。肌样细胞产生的比力是对照成年啮齿动物骨骼肌产生的比力的2-8%。从C2 C1/2 - 10 T 1/2细胞工程化的类肌细胞比从成年啮齿动物培养物工程化的类肌细胞表现出更大的基强度、达到峰值张力的时间和一半松弛时间，并且来自C2 C1/2 - 10 T 1/2和新生大鼠细胞的类肌细胞比来自成年啮齿动物培养物的类肌细胞具有更大的静息基线力。
The purpose of this study was to compare the excitability and contractility of three-dimensional skeletal muscle constructs, termed myooids, engineered from C2C12 myoblast and 10T 1/2 fibroblast cell lines, primary muscle cultures from adult C3H mice, and neonatal and adult Sprague-Dawley rats. Myooids were 12 mm long, with diameters of 0.1-1 mm, were excitable by transverse electrical stimulation, and contracted to produce force. After similar to 30 days in culture, myooid cross-sectional area, rheobase, chronaxie, resting baseline force, twitch force, time to peak tension, one-half relaxation time, and peak isometric force were measured. Specific force was calculated by dividing peak isometric force by cross-sectional area. The specific force generated by the myooids was 2-8% of that generated by skeletal muscles of control adult rodents. Myooids engineered from C2C12-10T 1/2 cells exhibited greater rheobase, time to peak tension, and one-half relaxation time than myooids engineered from adult rodent cultures, and myooids from C2C12-10T 1/2 and neonatal rat cells had greater resting baseline forces than myooids from adult rodent cultures.