Activation of metabotropic glutamate receptors is necessary for transneuronal regulation of ribosomes in chick auditory neurons
Activation of metabotropic glutamate receptors is necessary for transneuronal regulation of ribosomes in chick auditory neurons
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DOI：
10.1016/s0006-8993(98)00873-7
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发表时间：
1998-11-02
期刊：
影响因子：
2.9
通讯作者：
Hyson, RL
中科院分区：
文献类型：
--
作者：
Hyson, RL
Elimination of auditory nerve activity results in atrophy and death of nucleus magnocellularis (NM) neurons in the chick. One early event in the degeneration of NM neurons is a disruption of their ribosomes. This experiment examines the role of metabotropic glutamate receptors in afferent regulation of ribosomes. The auditory nerve on one side of a chick brainstem slice was stimulated in vitro. Rapid stimulation-dependent changes in ribosomes were visualized by immunolabeling using an antibody, called Y10B, that recognizes ribosomal RNA. In normal media, NM neurons on the stimulated side of the slice show greater Y10B labeling than the unstimulated NM neurons on the opposite side of the same slice. The role of metabotropic glutamate receptors was evaluated by unilaterally stimulating the auditory nerve in media containing the metabotropic glutamate receptor antagonist (RS)-alpha-methyl-4-carboxyphenyl-glycine (MCPG). Addition of MCPG to the bath did not block EPSPs produced by stimulating the auditory nerve. However, MCPG did prevent the stimulation-dependent regulation of ribosomes in NM neurons (as indexed by Y10B labeling). These data suggest that glutamate may play a trophic role in the young auditory system through activation of metabotropic glutamate receptors. (C) 1998 Elsevier Science B.V. All rights reserved.
