Activation of human immunodeficiency virus type 1 by oxidative stress.
Activation of human immunodeficiency virus type 1 by oxidative stress.
复制标题
氧化应激激活 1 型人类免疫缺陷病毒。
DOI：
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发表时间：
1990
影响因子：
1.5
通讯作者：
J. Piette
中科院分区：
文献类型：
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作者：
S. Legrand;D. Vaira;J. Pincemail;A. Vorst;J. Piette
1 型人类免疫缺陷病毒 (HIV-1) 感染的一个重要方面是其临床潜伏期，这表明病毒本身或原病毒可能在初次感染后长时间保持潜伏状态。某些异源病毒蛋白或化学和物理试剂能够重新激活潜伏病毒。由于这些药物的共同点是能够在细胞中引起应激反应，因此我们研究了过氧化氢 (H2O2) 介导的氧化应激对 HIV-1 潜伏感染的幼单核细胞系 U1 的影响。暴露于浓度范围为 0.1 至 2 mM 的 H2O2 后，U1 细胞的活力在恢复前 24 小时内下降。应激后 24 小时，根据培养物上清液中逆转录酶活性升高的情况评估，U1 细胞开始表达病毒。使用抗 HIV-1 多克隆抗体对受应激的 U1 细胞进行免疫荧光显示，H2O2 会导致 HIV-1 基因表达激活，但不会导致受损细胞释放病毒颗粒。此外，使用含有整合的细菌氯霉素乙酰转移酶（CAT）基因并受HIV-1长末端重复序列（LTR）控制的HeLa细胞系，我们发现H2O2介导的氧化应激允许细胞内CAT活性揭示的病毒LTR反式激活。当这些细胞用 0.5 mM H2O2 处理时，可以达到 CAT 活性约 4 的刺激因子。（摘要截断为 250 字）
An important aspect of the infection by the human immunodeficiency virus (HIV-1) type 1 is its clinical latency, suggesting that the virus itself or the provirus may remain latent for extended periods of time after primary infection. Certain heterologous viral proteins or chemical and physical agents are able to reactivate latent virus. Since a common denominator shared by these agents is the ability to cause stress response in cells, we have examined the effects of oxidative stress mediated by hydrogen peroxide (H2O2) on HIV-1 latently infected promonocytic cell line termed U1. After exposure to H2O2 in concentrations ranging from 0.1 to 2 mM, the viability of the U1 cells decreased during 24 h before recovery. At 24 h post stress, the U1 cells began to express virus as assessed by elevated reverse transcriptase activities in culture supernatants. Immunofluorescence carried out on stressed U1 cells using anti-HIV-1 polyclonal antibodies showed that H2O2 leads to HIV-1 gene expression activation, but not to a release of viral particles from damaged cells. Additionally, using a HeLa cell line containing integrated the bacterial chloramphenicol acetyl transferase (CAT) gene under the control of the HIV-1 long terminal repeat (LTR), we have shown that oxidative stress mediated by H2O2 allows transactivation of the viral LTR revealed by intracellular CAT activity. A stimulation factor of around 4 of CAT activity can be reached when these cells are treated with 0.5 mM H2O2.(ABSTRACT TRUNCATED AT 250 WORDS)
中性粒细胞髓过氧化物酶系统对氧化膜的损伤。
DOI：
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发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Sepe,SM;Clark,RA
通讯作者：
Clark,RA
影响因子：
15.9
作者：
SCHRAUFSTATTER, I;HYSLOP, PA;COCHRANE, CG
通讯作者：
COCHRANE, CG
DOI：
10.1073/pnas.86.24.9697
发表时间：
1989-12-01
影响因子：
11.1
作者：
SHIGENAGA, MK;GIMENO, CJ;AMES, BN
通讯作者：
AMES, BN