A role for nyctalopin, a small leucine-rich repeat protein, in localizing the TRP melastatin 1 channel to retinal depolarizing bipolar cell dendrites.
A role for nyctalopin, a small leucine-rich repeat protein, in localizing the TRP melastatin 1 channel to retinal depolarizing bipolar cell dendrites.
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Nyctalopin（一种富含亮氨酸的小重复蛋白）在将 TRP Melastatin 1 通道定位到视网膜去极化双极细胞树突中的作用
DOI：
10.1523/jneurosci.1014-11.2011
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发表时间：
2011-07-06
期刊：
影响因子：
--
通讯作者：
Gregg RG
中科院分区：
文献类型：
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作者：
Pearring JN;Bojang P Jr;Shen Y;Koike C;Furukawa T;Nawy S;Gregg RG
特定神经元部位的通道表达可以严重影响它们的功能和调节。目前，瞬时受体潜力(Trp)通道靶向和细胞内转运的分子机制仍不清楚，识别参与这些过程的蛋白质将有助于深入了解潜在的机制。视觉依赖于视网膜去极化双极细胞(DBCS)的正常功能，DBCS将代谢性谷氨酸受体6连接到Trp Melastatin 1(TRPM1)通道来传递来自光感受器的信号。我们报道了胞外膜附着蛋白Nyctalopin是小鼠DBCS树突上TRPM1正常表达所必需的。生化和遗传学数据表明，Nyctalopin和TRPM1直接相互作用，这表明Nyctalopin是辅助Trp通道亚单位，对正确定位到突触的通道至关重要。
Expression of channels to specific neuronal sites can critically impact their function and regulation. Currently, the molecular mechanisms underlying this targeting and intracellular trafficking of transient receptor potential (TRP) channels remain poorly understood, and identifying proteins involved in these processes will provide insight into underlying mechanisms. Vision is dependent on the normal function of retinal depolarizing bipolar cells (DBCs), which couple a metabotropic glutamate receptor 6 to the TRP melastatin 1 (TRPM1) channel to transmit signals from photoreceptors. We report that the extracellular membrane-attached protein nyctalopin is required for the normal expression of TRPM1 on the dendrites of DBCs in mus musculus. Biochemical and genetic data indicate that nyctalopin and TRPM1 interact directly, suggesting that nyctalopin is acting as an accessory TRP channel subunit critical for proper channel localization to the synapse.