Evaluation of raw nepodin extraction from Rumex japonicus and R. obtusifolius and their DNA polymorphisms.
Evaluation of raw nepodin extraction from Rumex japonicus and R. obtusifolius and their DNA polymorphisms.
复制标题
酸模和钝叶酸模中粗新孢素的提取及其 DNA 多态性的评价。
DOI：
10.1007/s11418-017-1143-0
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Woo JT.
中科院分区：
文献类型：
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作者：
Minami M;Mori T;Yonezawa T;Saito Y;Teruya T;Woo JT.
Nepodin，存在于酸模的根部。 （蓼科），抑制破骨细胞分化并具有抗糖尿病作用。我们建议将尼波丁作为新型功能性食品的成分或作为候选药物，以降低骨质疏松等疾病导致的运动能力下降的风险。虽然之前没有R的报道。 obtusifoliusL.在日本各地都有发现，其根部含有nepodin，我们在该物种的根中发现了nepodin。因此，R. obtusifoliusas 以及 R.日本刺参被认为是提取新波碱的候选原料。我们还讨论了R的适用性。日本刺参obtusifolius 是在琉球群岛种植的原始 nepodin 的来源。在这项研究中，琉球群岛上的所有标本均被鉴定为R。日本刺参。相反，日本大陆的所有标本都是R。钝叶。叶绿体trnL-trnF基因间间隔区和部分核内转录间隔区的DNA序列与R的鉴定一致。日本刺参obtusifolius通过花被片的形态特征。因此，为了避免错误识别和误用用于提取原料的植物物种，最好为这两个区域开发DNA标记。 R 中尼波丁的含量从检测不到到鲜重 (%FW) 的 0.34% 不等。日本刺参从不可检测到 R 中的 0.21%FW。钝叶。从药理学角度来看，作为可能适合作为尼波丁提取原料的植物，很明显，两种R.日本刺参obtusifolius 可以以相同的预期提取效率使用。根据我们的发现，R.无法证实obtusifolius 栖息于琉球群岛。为此，要保护琉球群岛特有的遗传特征，防止琉球群岛的遗传污染。钝叶，仅R.刺参应在琉球群岛种植。
Nepodin, found in the roots ofRumex japonicusHoutt. (Polygonaceae), inhibits osteoclast differentiation and has an antidiabetic effect. We propose nepodin as an ingredient of new functional foods or as a drug candidate for reducing the risk of reduced locomotion resulting from diseases such as osteoporosis. Although there are no previous reports ofR. obtusifoliusL., which is found throughout Japan, having roots containing nepodin, we found nepodin in the roots of this species. Therefore,R. obtusifoliusas well asR. japonicuswas considered a candidate raw material for nepodin extraction. We also discuss the suitability ofR. japonicusandR. obtusifoliusas sources of raw nepodin for cultivation on the Ryukyu Islands. In this study, all specimens on the Ryukyu Islands were identified asR. japonicus. Conversely, all specimens on mainland Japan wereR. obtusifolius. The DNA sequence of the chloroplasttrnL–trnF intergenic spacer region and partial nuclear internal transcribed spacer was consistent with the identification ofR. japonicusandR. obtusifoliusby morphological characteristics of the perianth segments. Therefore, to avoid erroneous identification and misuse of the plant species used for extraction of raw materials, it is preferable to develop DNA markers for these two regions. The content of nepodin varied from undetectable to 0.34% of the fresh weight (%FW) inR. japonicusand from undetectable to 0.21%FW inR. obtusifolius. From a pharmacological perspective, as plants that might be suitable as raw materials for nepodin extraction, it became clear that bothR. japonicusandR. obtusifoliuscan be used with the same expected extraction efficiency. Based on our findings,R. obtusifoliuscould not be confirmed as inhabiting the Ryukyu Islands. For this reason, to conserve the endemic genetic characteristics of the Ryukyu Islands and to prevent genetic pollution byR. obtusifolius,onlyR. japonicusshould be cultivated on the Ryukyu Islands.