Cdc42 regulates LPS-induced proliferation of primary pulmonary microvascular endothelial cells via ERK pathway
Cdc42 regulates LPS-induced proliferation of primary pulmonary microvascular endothelial cells via ERK pathway
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Cdc42通过ERK通路调节LPS诱导的原代肺微血管内皮细胞增殖
DOI：
10.1016/j.mvr.2016.10.001
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发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Chen, Yinghua
中科院分区：
文献类型：
--
作者：
Lv, Jiawen;Zeng, Junchao;Chen, Yinghua
工作背景：细胞分裂周期蛋白42（Cell Division Cycle Protein 42，Cdc 42）是一种重要的细胞分裂周期蛋白，在损伤后通过增强细胞间粘附连接来恢复和增强屏障功能，但其对损伤后细胞增殖的影响尚不清楚。我们用不同剂量的LPS刺激PMVEC，并评估对细胞增殖的影响。我们还构建了一个原代缺失Cdc42基因的细胞系，以验证Cdc42在调节LPS刺激的PMVEC增殖中的作用，并探讨相关的信号通路。结论：Cdc42参与了小剂量LPS刺激的PMVECs增殖的调控过程，该过程通过ERIC途径参与。(C)2016 Elsevier Inc. All rights reserved.
Background: After stimulation due to injury, cell division cycle protein 42 (Cdc42) restores and enhances barrier functions by strengthening intercellular adherens junctions; however, its influence on cell proliferation after injury remains unknown.Objective: In this study, we sought to investigate the effect of stimulation using small doses of lipopolysaccharide (LPS) on the proliferation of pulmonary microvascular endothelial cells (PMVECs).Methods: We stimulated PMVECs with different doses of LPS and evaluated the effects on cell proliferation. We also constructed a primary gene-knockout cell line lacking Cdc42 to verify the role of Cdc42 in regulating the proliferation of PMVECs that were stimulated using LPS and to explore related signaling pathways.Results: Stimulating PMVECs with small doses of LPS increased proliferation. Cdc42 is involved in regulating this process, which was mediated by the extracellular regulated protein kinase (ERK) pathway.Conclusions: Cdc42 plays a role in regulating the proliferation of PMVECs stimulated with small doses of LPS, and this regulation involves the ERIC pathway. (C) 2016 Elsevier Inc. All rights reserved.