Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated Strain
Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated Strain
复制标题
DOI：
10.1155/2013/918320
复制
发表时间：
2013-01-01
影响因子：
--
通讯作者：
Li, Huiying
中科院分区：
文献类型：
--
作者：
Kasimatis, Gabriela;Fitz-Gibbon, Sorel;Li, Huiying
人类皮肤中含有多种细菌，包括革兰氏阳性厌氧菌痤疮丙酸杆菌。痤疮丙酸杆菌历来与寻常痤疮的发病机制有关，寻常痤疮是一种常见的皮肤病，影响美国超过80%的青少年。为了深入了解潜在的痤疮丙酸杆菌致病机制，我们先前对与痤疮高度相关的痤疮丙酸杆菌菌株HL 096 PA 1的全基因组进行了测序。在这项研究中，我们将其基因组与第一个公布的完整基因组KPA 171202进行了比较。HL 096 PA 1含有线性质粒pIMPLE-HL 096 PA 1。这是第一个描述的痤疮丙酸杆菌质粒。我们还观察到HL 096 PA 1中的假基因增加了5倍，其中一些编码碳水化合物转运和代谢蛋白。此外，我们的分析揭示了一些岛状的基因组区域是独特的HL 096 PA 1和一个大的基因组倒位跨越核糖体操纵子。总之，这些发现为理解痤疮丙酸杆菌的毒力特性、宿主适应机制及其在菌株水平上在痤疮发病机制中的潜在作用提供了基础。此外，在HL 096 PA 1中鉴定的质粒可能为痤疮丙酸杆菌的遗传操作和针对特定疾病相关菌株的靶向治疗提供新的机会。
The human skin harbors a diverse community of bacteria, including the Gram-positive, anaerobic bacterium Propionibacterium acnes. P. acnes has historically been linked to the pathogenesis of acne vulgaris, a common skin disease affecting over 80% of all adolescents in the US. To gain insight into potential P. acnes pathogenic mechanisms, we previously sequenced the complete genome of a P. acnes strain HL096PA1 that is highly associated with acne. In this study, we compared its genome to the first published complete genome KPA171202. HL096PA1 harbors a linear plasmid, pIMPLE-HL096PA1. This is the first described P. acnes plasmid. We also observed a five-fold increase of pseudogenes in HL096PA1, several of which encode proteins in carbohydrate transport and metabolism. In addition, our analysis revealed a few island-like genomic regions that are unique to HL096PA1 and a large genomic inversion spanning the ribosomal operons. Together, these findings offer a basis for understanding P. acnes virulent properties, host adaptation mechanisms, and its potential role in acne pathogenesis at the strain level. Furthermore, the plasmid identified in HL096PA1 may potentially provide a new opportunity for P. acnes genetic manipulation and targeted therapy against specific disease-associated strains.