Expression of membrane-bound transferrin-like protein p97 on the cell surface of chondrocytes.
Expression of membrane-bound transferrin-like protein p97 on the cell surface of chondrocytes.
复制标题
膜结合转铁蛋白样蛋白 p97 在软骨细胞表面的表达。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Y. Kato
中科院分区：
文献类型：
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作者：
T. Kawamoto;H. Pan;W. Yan;H. Ishida;E. Usui;R. Oda;K. Nakamasu;M. Noshiro;Y. Kawashima;M. Fujii;H. Shintani;Y. Okada;Y. Kato
从兔软骨细胞培养物的质膜部分中纯化出 76 kDa 的伴刀豆球蛋白 A 结合蛋白。该蛋白的 N 末端区域和胰蛋白酶肽的氨基酸测序以及其 cDNA 的测序表明该蛋白与肿瘤相关抗原 p97 高度相似。因此，可以得出结论，该蛋白是p97的兔形式。 Western blotting、Northern blotting和逆转录PCR分析表明，兔p97在软骨和软骨细胞中高水平表达，但在骨、肝、肾、小肠、眼、胰腺、心脏、睾丸、骨骼肌、脾和成纤维细胞中几乎检测不到。免疫细胞化学和免疫组织化学分析表明p97在软骨细胞的质膜中表达。在软骨的所有区域中均检测到 p97 转录物，但在肥大区域中水平相对较低。这些发现表明 p97 参与维持软骨细胞的细胞表面特征。
A concanavalin-A-binding protein of 76 kDa was purified from the plasma membrane fraction of rabbit chondrocyte cultures. Amino acid sequencing of the N-terminal region and of tryptic peptides of the protein, in addition to sequencing of its cDNA revealed that this protein is highly similar to the tumour-associated antigen p97. Hence, it was concluded that this protein is the rabbit form of p97. Western blotting, Northern blotting and reverse-transcription PCR analyses indicated that rabbit p97 is expressed at high levels in cartilage and chondrocytes, but is barely detectable in the bone, liver, kidney, small intestine, eye, pancreas, heart, testis, skeletal muscle, spleen and fibroblasts. Immunocytochemical and immunohistochemical analyses demonstrated that p97 is expressed in the plasma membrane of chondrocytes. p97 transcript was detected in all zones of the cartilage but the level was relatively low in the hypertrophic zone. These findings suggest that p97 is involved in maintaining the cell surface characteristics of chondrocytes.