An enzyme immunoassay for determining plasma concentrations of didemnin B.
An enzyme immunoassay for determining plasma concentrations of didemnin B.
复制标题
一种酶联免疫测定法，用于测定地德明宁 B 的血浆浓度。
DOI：
10.1002/jcla.1860060307
复制
发表时间：
1992
影响因子：
2.7
通讯作者：
Newman,RA
中科院分区：
文献类型：
--
作者：
Raybould,TJ;Grothaus,PG;Simpson,SB;Bignami,GS;Lazo,CB;Newman,RA
Didemnin A was conjugated at the amino terminus of theN‐methylleucine residue, via the linkersN‐succinimidyl‐3‐(2‐pyridyldithio)propionate and trans‐1,4‐maleimidomethylcyclohexane carboxylic acid, to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA). The didemnin‐KLH conjugates were used to hyperimmunize rabbits. The resulting high titer antisera were employed with didemnin‐BSA conjugate‐coated microtiter plate wells to develop an indirect competitive inhibition enzyme immunoassay (CIEIA) that was fully cross reactive with didemnin B. A CIEIA is described that is capable of detecting the drug in plasma from didemnin Btreated patients at concentrations down to 1‐3 ng/ml. This simple, sensitive CIEIA has been employed to demonstrate plasma drug clearance profiles with samples from didemnin B‐treated patients. © 1992 Wiley‐Liss, Inc.
