Genome-wide association study identifies new loci for 1000-seed weight in Brassica napus
Genome-wide association study identifies new loci for 1000-seed weight in Brassica napus
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DOI：
10.1007/s10681-022-03097-7
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发表时间：
2022-09
期刊：
影响因子：
1.9
通讯作者：
Haijiang Liu;Jing Wang;Bingbing Zhang;Xinyu Yang;Pan Yuan;G. Ding;Sheliang Wang;H. Cai;
中科院分区：
文献类型：
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作者：
Haijiang Liu;Jing Wang;Bingbing Zhang;Xinyu Yang;Pan Yuan;G. Ding;Sheliang Wang;H. Cai;
油菜（Brassica napusL.）油菜是世界上重要的油料作物，而千粒重是决定油菜种子产量的重要因素。油菜。为了阐明SW的遗传机制和挖掘候选基因，一组403个不同的B。在全基因组关联研究（GWAS）中，使用160万个单核苷酸多态性（SNP）筛选了100份油菜种质。本研究通过一般线性模型（GLM）和混合线性模型（MLM）筛选出340个与SW显著相关的SNPs。通过GWAS结合转录组数据，筛选出两个差异表达显著的基因（BnaA02g06870D和BnaC06g28920D）作为候选基因。候选基因关联分析和单倍型分析表明，携带ACCC atBnaA02g06870Hap1和TTGG atBnaC06g28920Hap1的自交系比携带其他单倍型等位基因的自交系具有更大的SW。本研究所确定的候选基因和有利单倍型将有助于大粒种选育。油菜。
Oilseed rape (Brassica napusL.) is an important oilseed crop worldwide and 1000-seed weight (SW) is the important determinant of seed yield ofB. napus. To elucidate the genetic mechanism of SW and mining candidate genes, a panel of 403 diverseB. napusaccessions was screened in a genome-wide association study (GWAS) using 1.60 million single-nucleotide polymorphisms (SNPs). This study identified 340 SNPs significantly associated with SW by general linear model (GLM) and mixed linear model (MLM). Through GWAS combined with transcriptome data, two significantly differentially expressed genes were identified as candidate genes (BnaA02g06870DandBnaC06g28920D). Candidate gene association analysis and haplotype analysis showed that the inbred lines carrying ACCC atBnaA02g06870Hap1and TTGG atBnaC06g28920Hap1had greater SW than lines carrying other haplotype alleles. Candidate genes and favourable haplotypes identified in this study will be useful for large-seed breeding ofB. napus.