Expression of nerve growth factor (NGF), and its receptors TrkA and p75 in the reproductive organs of the adult male rats
Expression of nerve growth factor (NGF), and its receptors TrkA and p75 in the reproductive organs of the adult male rats
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DOI：
10.2108/zsj.22.933
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发表时间：
2005-08-01
影响因子：
0.9
通讯作者：
Taya, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Li, ChunMei;Watanabe, Gen;Taya, Kazuyoshi
本文研究了神经生长因子(NGF)及其受体TrkA和p75在成年雄性大鼠生殖器官中的免疫定位。用免疫组织化学ABC法对成年雄性大鼠的睾丸、传出管、附睾管、输精管、精囊、凝固腺和前列腺组织切片进行免疫组织化学染色。NGF表达于睾丸的间质细胞、初级精母细胞和粗线型精母细胞。TrkA仅对细长精子细胞有免疫反应，而p75在支持细胞、间质细胞、粗线期精母细胞和细长精子细胞中呈阳性反应。在传出管、输精管和附睾管上皮细胞中检测到NGF及其两种受体的免疫反应。此外，在精囊、前列腺和凝固腺的柱状分泌上皮系中也观察到NGF及其两种受体的免疫反应。这些结果提示，NGF是成年雄性大鼠性腺功能的重要生长因子。
Immunolocalization of nerve growth factor (NGF) and its receptors, TrkA and p75 in the reproductive organs of adult male rats was investigated. Sections of the testis, efferent duct, epididymis, deferent duct, seminal vesicle, coagulating gland and prostate of adult male rats were immunostained by the avidin-biotin-peroxidase complex methods (ABC). NGF was expressed in Leydig cells, primary spermatocytes and pachytene spermatocytes in the testis. TrkA only immunoreacted to elongate spermatids and p75 showed positive immunostaining in the Sertoli cells, Leydig cells, the pachytene spermatocytes and elongate spermatids. Immunoreactions for NGF and its two receptors were detected in epithelial cells of efferent duct, deferent duct and epididymis. In addition, immunoreactions for NGF and its two receptors were also observed in columnar secretory epithelium lines of the seminal vesicles, prostate and coagulating gland. These results suggest that NGF is an important growth factor in gonadal function of adult male rats.