A pilot study of transplantation of an autologous corneal epithelial cell sheet in a canine model of corneal injury
A pilot study of transplantation of an autologous corneal epithelial cell sheet in a canine model of corneal injury
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发表时间：
2018-05
影响因子：
0.4
通讯作者：
E. Nam;N. Fujita;Maresuke Morita;H. Lin;Kentaro Endo;T. Nakagawa;R. Nishimura;K. Tsuzuki
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作者：
E. Nam;N. Fujita;Maresuke Morita;H. Lin;Kentaro Endo;T. Nakagawa;R. Nishimura;K. Tsuzuki
角膜移植是恢复角膜透明性的最有效方法。然而，由于免疫反应可能发生移植排斥，并阻止受伤的角膜表面的适当愈合。为了解决这个问题，我们将自体角膜上皮片移植到狗的受伤角膜表面，并评估该片的临床使用效率和安全性。四只角膜表面受伤的狗-三只接受移植角膜上皮细胞片的实验狗和一只没有接受移植的对照狗。我们从每只狗身上去除了整个角膜上皮层和浅层基质。从自体角膜缘段培养角膜上皮片，并在去除三只狗的角膜表面后21天移植。未接受移植的犬在接受角膜损伤后60天被安乐死。移植组于移植后60天取犬角膜。结果：移植组角膜恢复透明。移植组中的一只犬对缝线表现出免疫反应。移植组术后60天角膜混浊和新生血管形成可忽略不计。此外，对照犬显示角膜表面不透明。移植区域均显示上皮细胞过度增殖和间质细胞过多。愈合角膜表面的干细胞/祖细胞标记也显示出与正常角膜相似的模式。我们相信自体薄片的移植可以恢复角膜的透明度，并防止严重损伤引起的不可逆的混浊。
Corneal transplantation is the most effective method of restoring corneal transparency. However, graft rejection due to immunoreaction may occur and prevent proper healing of the wounded corneal surface. To address this issue, we transplanted an autologous corneal epithelial sheet into the wounded corneal surface of a dog and evaluated the sheet’s efficiency and safety for clinical use. Four dogs with a wounded corneal surface— three experimental dogs receiving transplanted corneal epithelial cell sheets and a control dog who did not receive the transplant. We removed the entire layers of corneal epithelium and superficial stroma from each dog. Corneal epithelial sheets were cultivated from autologous limbal segments and were transplanted 21 days after removing the three dogs’ corneal surfaces. The dog that did not undergo transplantation was euthanized 60 days after receiving corneal injury. The corneas of dogs in the transplantation group were collected 60 days after the transplant. Results: Corneal transparency was restored in the transplantation group. One dog in the transplantation group showed immunoreaction to the sutures. However, corneal opacity and neovascularization were negligible 60 days after the transplant in the transplantation group. In addition, the control dog showed opacity of the corneal surface. The transplanted areas each showed hyperproliferation in the epithelium and hypercellularity in the stroma. The stem/progenitor markers for a healing corneal surface also showed patterns similar to a normal cornea. We believe that the transplantation of an autologous sheet may restore corneal transparency and prevent irreversible opacity caused by severe injury.