Distribution of culturable microorganisms in Fennoscandian Shield groundwater
Distribution of culturable microorganisms in Fennoscandian Shield groundwater
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DOI：
10.1111/j.1574-6941.2002.tb00914.x
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发表时间：
2002-02-01
影响因子：
4.2
通讯作者：
Pedersen, K
中科院分区：
文献类型：
--
作者：
Haveman, SA;Pedersen, K
对芬兰和瑞典6个芬诺斯坎迪亚盾构点的16个地下水样品中的微生物种群进行了调查。平均细胞总数为3.7×10~(-5)细胞·ml~(-1)，总细胞数平均值在1390m处没有变化。根据每个地下水样品的化学成分设计了培养基并成功地用于65~1350m深度的所有样品的厌氧微生物培养。细胞总数的0.0084%~14.8%来自地下水样本。硫酸盐还原细菌、铁还原细菌和异养产乙酸菌是从地理位置较远的65-686m深度的地下水中培养出来的。从更深、更老、更咸的地下水中培养出不同的微生物种群，深度从863到1350。对地下水化学数据的主成分分析表明，大部分咸水中未检测到硫酸盐还原细菌和铁还原菌。铁还原细菌和乙酸菌是从含0.35-3.5 mm硫酸盐的深层地下水中培养出来的，产甲烷菌和乙酸菌则是从硫酸盐缺乏的深层地下水中培养出来的。在培养自养产甲烷菌的一个井里。与Fennoscandian Shield的其他地下水样品相比，溶解的无机碳在C-13中富含，这表明自养生物是活跃的。可以得出结论，在芬诺斯坎迪亚盾内，从地表到1300米深处都存在着多样化的微生物群落。(C)2002年欧洲微生物学会联合会。爱思唯尔科学公司出版。版权所有。
Microbial populations in 16 groundwater samples from six Fennoscandian Shield sites in Finland and Sweden were investigated. The average total cell number was 3.7 X 10(5) cells ml(-1), and there was no change in the mean of the total cell numbers to a depth of 1390 m. Culture media were designed based on the chemical composition of each groundwater sample and used successfully to culture anaerobic microorganisms from all samples between 65 and 1350 m depth. Between 0.0084 and 14.8% of total cells were cultured from groundwater samples. Sulfate-reducing bacteria, iron-reducing bacteria and heterotrophic acetogenic bacteria were cultured from groundwater sampled at 65-686 m depth in geographically distant sites. Different microbial populations were cultured from deeper, older and more saline groundwater from 863 to 1350 in depth. Principal component analysis of groundwater chemistry data showed that sulfate- and iron-reducing bacteria were not detected in the most saline groundwater. Iron-reducing bacteria and acetogens were cultured from deep groundwater that contained 0.35-3.5 mM sulfate, while methanogens and acetogens were cultured from deep sulfate-depleted groundwater. In one borehole from which autotrophic methanogens were cultured. dissolved inorganic carbon was enriched in C-13 compared to other Fennoscandian Shield groundwater samples, suggesting that autotrophs were active. It can be concluded that a diverse microbial community is present from the surface to over 1300 m depth in the Fennoscandian Shield. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.