Association between intraepithelial Escherichia coli and colorectal cancer
Association between intraepithelial Escherichia coli and colorectal cancer
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DOI：
10.1016/s0016-5085(98)70194-5
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发表时间：
1998-08-01
期刊：
影响因子：
29.4
通讯作者：
Lochs, H
中科院分区：
文献类型：
--
作者：
Swidsinski, A;Khilkin, M;Lochs, H
背景与目的：虽然许多研究都集中在幽门螺杆菌，但对结肠粘膜相关植物群知之甚少。本研究的目的是直接检测大肠癌筛查患者结肠粘膜中的细菌。研究方法：细菌定量与聚合酶链反应和比较序列分析确定在结肠镜活检标本31例无症状和34例有症状的对照正常结肠镜检查结果，29例结肠腺瘤，31例结直肠癌。在41例患者中，用庆大霉素保护试验证实了细菌的细胞内和细胞外位置。结果：97%的无症状患者和69%的有症状对照组的活检标本中未检出细菌。相比之下，90%的腺瘤和93%的癌患者的恶性组织和肉眼可见的正常组织的活检标本中分别检测到10（3）-10（5）个菌落形成单位/微升的细菌浓度。E.大肠杆菌和大肠杆菌样细菌在这些患者中的82%的结肠粘膜中定植。庆大霉素保护试验表明，E.大肠杆菌在87%的腺瘤和癌患者中部分位于细胞内，而在对照组中没有。结论：大肠癌患者结肠粘膜中存在大肠杆菌的定植，而正常结肠粘膜中无大肠杆菌定植。杆菌
Background & Aims: Although multiple studies have focused on Helicobacter pylori, little is known about the mucosa-associated flora of the colon. The aim of this study was to detect bacteria directly in colonic mucosa from patients screened for colorectal cancer. Methods: Bacteria were quantified with the polymerase chain reaction and identified by comparative sequence analysis in colonoscopic biopsy specimens from 31 asymptomatic and 34 symptomatic controls with normal colonoscopic findings, 29 patients with colonic adenoma, and 31 patients with colorectal carcinoma. In 41 patients, intra- and extracellular location of bacteria was confirmed with the gentamicin protection assay. Results: No bacteria were detected in biopsy specimens from 97% of asymptomatic and 69% of symptomatic controls. In contrast, bacterial concentrations of 10(3)-10(5) colony-forming units per microliter were detected in biopsy specimens from both malignant and macroscopically normal tissue in 90% and 93% of patients with adenoma and carcinoma, respectively. E. coli and coli-like bacteria were shown to colonize the colonic mucosa in 82% of these patients. The gentamicin protection assay indicated that E. coli was partially intracellular in 87% of patients with adenoma and carcinoma and in none of the controls. Conclusions: The colonic mucosa of patients with colorectal carcinoma but not normal colonic mucosa is colonized by intracellular E. coli.