Evaluation of microencapsulation of the UFV-AREG1 bacteriophage in alginate-Ca microcapsules using microfluidic devices
Evaluation of microencapsulation of the UFV-AREG1 bacteriophage in alginate-Ca microcapsules using microfluidic devices
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DOI：
10.1016/j.colsurfb.2017.06.045
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发表时间：
2017-10-01
影响因子：
5.8
通讯作者：
Mendonca, Regina C. S.
中科院分区：
文献类型：
--
作者：
Boggione, Delaine M. G.;Batalha, Lais S.;Mendonca, Regina C. S.
抗生素的滥用和耐药微生物的出现已成为食品工业面临的主要挑战。本工作的目的是使用微流体装置将噬菌体UFV-AREG 1微囊化在藻酸钙基质中，并研究保留的活力和效率。将微胶囊添加到丙二醇的凝胶中用作食品工业中的抗微生物剂。该技术显示了噬菌体包封的数量，产生平均直径为100-250 μ m的液滴，82.1 +/-2%的保留效率和在凝胶基质中21天的稳定性。添加到微囊化噬菌体中的凝胶显示出以与抗微生物化学品（酒精70%）相似的水平减少表面上的细菌污染的效率（在表面上不可检测）。因此，有可能将噬菌体微囊化在藻酸盐-Ca中，并将微胶囊应用于凝胶中，用作食品工业中的消毒剂。(C)2017爱思唯尔B. V.保留所有权利。
The indiscriminate use of antibiotics and the emergence of resistant microorganisms have become a major challenge for the food industry. The purpose of this work was to microencapsulate the bacteriophage UFV-AREG1 in a calcium alginate matrix using microfluidic devices and to study the viability and efficiency of retention. The microcapsules were added to gel of propylene glycol for use as an antimicrobial in the food industry. The technique showed the number of the phage encapsulation, yielding drops with an average 100-250 mu m of diameter, 82.1 +/- 2% retention efficiency and stability in the gel matrix for 21 days. The gel added to the microencapsulated phage showed efficiency (not detectable on the surface) in reducing bacterial contamination on the surface at a similar level to antimicrobial chemicals (alcohol 70%). Therefore, it was possible to micro encapsulate bacteriophages in alginate-Ca and apply the microcapsules in gels for use as sanitizers in the food industry. (C) 2017 Elsevier B.V. All rights reserved.