Ectopic gene expression and homeotic transformations in arthropods using recombinant Sindbis viruses
Ectopic gene expression and homeotic transformations in arthropods using recombinant Sindbis viruses
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DOI：
10.1016/s0960-9822(00)80049-4
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发表时间：
1999-11-18
期刊：
影响因子：
9.2
通讯作者：
Carroll, SB
中科院分区：
文献类型：
--
作者：
Lewis, DL;DeCamillis, MA;Carroll, SB
背景：节肢动物形态的多样性使其成为研究发育机制进化的重要对象。比较分析表明，节肢动物多样性的出现主要是由于控制发育的基因表达模式的变化。由于缺乏广泛适用的基因表达调控技术，阻碍了对特定物种发育过程中特定基因功能的直接分析。我们报告说，虫媒病毒辛德毕斯可用于提供高水平的基因表达，在体内的一些非宿主节肢动物物种，而不会导致细胞病变的影响，在感染的细胞或损害发展。利用重组辛德毕斯病毒，我们研究了同源异型基因Ultrabithorax在蝴蝶翅膀和甲虫胚胎发育中的功能。在蝴蝶前翅成虫盘中异位表达的Ultrabithorax足以引起成虫特征性前翅性状（包括鳞片形态和色素沉着）向后翅性状的转化，超双胸基因在甲虫胚胎中的内源性表达域外的表达影响了体壁角质层和附属器的正常发育。长期以来，同源异型基因一直被认为在节肢动物附属物的多样化中发挥着重要作用。利用重组辛德比斯病毒，我们能够研究非模型节肢动物物种中的同源异型基因功能。我们发现，Ultrabithorax是足以赋予后翅身份的蝴蝶和改变正常发展的前结构的甲虫。重组辛德毕斯病毒作为分析节肢动物多样化过程中发育基因功能变化的工具具有广泛的潜力。
Background: The morphological diversity of arthropods makes them attractive subjects for studying the evolution of developmental mechanisms. Comparative analyses suggest that arthropod diversity has arisen largely as a result of changes in expression patterns of genes that control development, Direct analysis of how a particular gene functions in a given species during development is hindered by the lack of broadly applicable techniques for manipulating gene expression.Results: We report that the Arbovirus Sindbis can be used to deliver high levels of gene expression in vivo in a number of non-host arthropod species without causing cytopathic effects in infected cells or impairing development. Using recombinant Sindbis virus, we investigated the function of the homeotic gene Ultrabithorax in the development of butterfly wings and beetle embryos, Ectopic Ultrabithorax expression in butterfly forewing imaginal discs was sufficient to cause the transformation of characteristic forewing properties in the adult, including scale morphology and pigmentation, to those of the hindwing, Expression of Ultrabithorax in beetle embryos outside of its endogenous expression domain affected normal development of the body wall cuticle and appendages,Conclusions: The homeotic genes have long been thought to play an important role in the diversification of arthropod appendages, Using recombinant Sindbis virus, we were able to investigate homeotic gene function in non-model arthropod species. We found that Ultrabithorax is sufficient to confer hindwing identity in butterflies and alter normal development of anterior structures in beetles. Recombinant Sindbis virus has broad potential as a tool for analyzing how the function of developmental genes has changed during the diversification of arthropods.