NMR and circular dichroism studies of the lantibiotic nisin in non‐aqueous environments
NMR and circular dichroism studies of the lantibiotic nisin in non‐aqueous environments
复制标题
非水环境中羊毛硫抗生素乳链菌素的核磁共振和圆二色性研究
DOI：
10.1016/0014-5793(93)80065-3
复制
发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
F. van de Ven
中科院分区：
文献类型：
--
作者：
H. W. van den Hooven;F. Fogolari;H. Rollema;R. Konings;C. W. Hilbers;F. van de Ven
已知与某些革兰氏阳性细菌的膜相互作用的lantibiotic，nisin，在模拟膜样环境的三种模型系统中进行了研究，即三氟乙醇和水的混合物，或十二烷基硫酸钠或十二烷基磷酸胆碱的胶束。在40°C和pH 3.5的非水环境中，乳酸链球菌素的1H NMR谱已被完全指定。圆二色谱和核磁共振结果表明，乳链菌肽在三种非水环境中的构象与水溶液中的构象不同，而在两种胶束体系中的构象相似。相对于水溶液中的乳链菌肽，主要构象变化发生在N末端。
The lantibiotic, nisin, which is known to interact with membranes of certain Gram‐positive bacteria, was studied in three model systems which mimic a membrane‐like environment, i.e. a mixture of trifluoroethanol and water, or micelles of sodium dodecyl sulfate or dodecylphosphocholine. The1H NMR spectra of nisin in the non‐aqueous environments, at 40°C and pH 3.5, have been assigned completely. The CD and NMR results indicate that the conformation of nisin in the three non‐aqueous environments differs from that in aqueous solution, and that the conformation in the two micellar systems is similar. The major conformational changes, relative to nisin in aqueous solution, occur in the N‐terminus.
影响因子：
2.9
作者：
WISHART, DS;SYKES, BD;RICHARDS, FM
通讯作者：
RICHARDS, FM