Expression patterns of the transcription factor AP-2α during hair follicle morphogenesis and cycling
Expression patterns of the transcription factor AP-2α during hair follicle morphogenesis and cycling
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DOI：
10.1046/j.1523-1747.2003.12319.x
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发表时间：
2003-07-01
影响因子：
6.5
通讯作者：
Christiano, AM
中科院分区：
文献类型：
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作者：
Panteleyev, AA;Mitchell, PJ;Christiano, AM
AP-2α 是在上皮细胞和神经嵴谱系细胞中表达的转录因子家族的成员。 AP-2α 在胚胎发育和上皮基因转录调节中发挥重要作用。为了进一步表征 AP-2alpha 在皮肤生物学中的作用，我们评估了其在毛囊形态发生和循环的特定阶段在 C57BL/6J 小鼠皮肤中的表达。在早期毛囊形态发生过程中，AP-2α 在表皮基板、毛囊芽的基底角质形成细胞以及内根鞘中表达上调。在毛干形成开始和毛囊向下生长的过程中，毛囊乳头细胞经历了短暂的 AP-2α 表达上调。毛囊形态发生的完成与毛囊下部（包括上皮和间充质区室）AP-2α免疫反应性的显着降低相关。在青春期小鼠皮肤中，在表皮的基底角质形成细胞、毛囊漏斗部和皮脂细胞中发现了持续强的 AP-2α 表达。在毛囊乳头中，AP-2α在休止期表达较弱，在毛发生长初期显着上调，随后逐渐下降，在毛发生长中期再次出现。在内根鞘中，在生长期早期和中期以及中期中期检测到 AP-2α 表达。在杵状毛发形成区域也观察到显着的 AP-2α 表达。因此，上皮毛囊室和间充质毛囊室中的 AP-2α 上调与主要重塑过程的启动相协调。我们的研究结果支持使用毛囊作为模型来探索 AP-2α 在发育器官的生理重塑和外胚层-间质相互作用中的作用。
AP-2alpha is a member of a family of transcription factors expressed in cells of the epithelial and neural crest lineage. AP-2alpha plays an essential role in embryonic development and in regulation of epithelial gene transcription. To further characterize the role of AP-2alpha in skin biology, we assessed its expression in the skin of C57BL/6J mice during defined stages of hair follicle morphogenesis and cycling. During early hair follicle morphogenesis, AP-2alpha was upregulated in the epidermal placode, in the basal keratinocytes of the hair follicle bud, and then in the inner root sheath. The follicular papilla cells underwent a brief upregulation of AP-2alpha expression during the initiation of hair shaft formation and active hair follicle downward growth. Completion of hair follicle morphogenesis was associated with a marked reduction of AP-2alpha immunoreactivity in the lower portion of the hair follicle including both epithelial and mesenchymal compartments. In adolescent mouse skin, consistently strong AP-2alpha expression was found in the basal keratinocytes of the epidermis, in the hair follicle infundibulum, and in the sebocytes. In the follicular papilla, AP-2alpha was weakly expressed in telogen, significantly upregulated in early anagen, then gradually declined, and reappeared again in middle catagen. In the inner root sheaths, AP-2alpha expression was detected during early and middle anagen and during middle catagen stages. Prominent AP-2alpha expression was also seen in the zone of club hair formation. Therefore, AP-2alpha upregulation in both epithelial and mesenchymal hair follicle compartments was coordinated with initiation of major remodeling processes. Our findings support the use of the hair follicle as a model to explore the role of AP-2alpha in physiologic remodeling of developing organs and in reciprocal ectodermal-mesenchymal interactions.