Creating a saturated reference map for the apple (Malus x domestica Borkh.) genome
Creating a saturated reference map for the apple (Malus x domestica Borkh.) genome
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DOI：
10.1007/s00122-003-1209-0
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发表时间：
2003-05-01
影响因子：
5.4
通讯作者：
Gessler, C.
中科院分区：
文献类型：
--
作者：
Liebhard, R.;Koller, B.;Gessler, C.
高质量连锁图谱的可用性对于数量性状的检测和分析至关重要。这样的图谱应该覆盖基因组的重要部分，应该密集地填充标记，并且为了获得最大的优势，应该可以转移到除其构建的群体或品种之外的群体或品种。苹果遗传连锁图谱是在品种 Fiesta 和 Discovery 杂交的分离群体的基础上构建的。总共840个分子标记，475个AFLP，235个RAPD，129个SSR和1个SCAR，用于用JoinMap构建的两个亲本图谱，跨度分别为1,140 cM和1,450 cM。大量的共显性标记，如 SSR，可以将图谱快速转移到其他种群或品种，从而可以研究另一个遗传背景中的任何选定性状。该图谱是目前苹果中基因组覆盖度和标记密度最先进的连锁图谱。它代表了 Malus 未来绘图项目的理想起点，因为地图的稳定且可转移的 SSR 框架可以快速被占主导地位的 AFLP 标记饱和。
The availability of a high quality linkage map is essential for the detection and the analysis of quantitative traits. Such a map should cover a significant part of the genome, should be densely populated with markers, and in order to gain the maximum advantage should be transferable to populations or cultivars other than the ones on which it has been constructed. An apple genetic linkage map has been constructed on the basis of a segregating population of the cross between the cultivars Fiesta and Discovery. A total of 840 molecular markers, 475 AFLPs, 235 RAPDs, 129 SSRs and 1 SCAR, were used for the two parental maps constructed with JoinMap and spanning 1,140 cM and 1,450 cM, respectively. Large numbers of codominant markers, like SSRs, enable a rapid transfer of the map to other populations or cultivars, allowing the investigation of any chosen trait in another genetic background. This map is currently the most advanced linkage map in apple with regard to genome coverage and marker density. It represents an ideal starting point for future mapping projects in Malus since the stable and transferable SSR frame of the map can be saturated quickly with dominant AFLP markers.