The surface structure of erythrocytes from some animal sources.
The surface structure of erythrocytes from some animal sources.
复制标题
某些动物来源的红细胞的表面结构。
DOI：
10.1016/0003-9861(63)90117-6
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发表时间：
1963
影响因子：
3.9
通讯作者：
G. Uhlenbruck
中科院分区：
文献类型：
--
作者：
G. Seaman;G. Uhlenbruck
它表明，蛋白水解酶降低人和黑猩猩红细胞的电泳迁移率，减少取决于特定酶的特异性。在人类红细胞的情况下，半定量相关性已被发现之间的这些减少和唾液酸的数量释放结合形式作为N-酰化神经氨酸含粘液。相反，蛋白水解酶对其他动物红细胞的作用，要么使电泳迁移率略有下降，要么没有变化，或者在鸡红细胞的情况下使电泳迁移率增加。用神经氨酸酶处理动物红细胞，在所有情况下都使动电电荷显著减少。鸡红细胞表面的唾液酸主要是N-乙酰神经氨酸，而马红细胞表面的唾液酸主要是N-羟乙酰神经氨酸，经神经氨酸酶、木瓜蛋白酶和胰蛋白酶连续酶处理后，发现不同种属红细胞表面的唾液酸有明显差异。
It is shown that proteolytic enzymes reduce the electrophoretic mobilities of human and chimpanzee erythrocytes, the decreases depending upon the specificity of the particular enzyme. In the case of human erythrocytes, a semiquantitative correlation has been found between these reductions and the quantities of sialic acids released in bound form asN-acylated neuraminic acid-containing mucoids. In contrast, the action of proteolytic enzymes on other animal erythrocytes produces either a small decrease, no change, or in the case of chicken red cells an increase in the electrophoretic mobility.Treatment of animal red cells with neuraminidase produces in all instances a marked reduction in the electrokinetic charge. The sialic acid present on the surface of chicken erythrocytes has been shown to be predominantlyN-acetylneuraminic acid while that from horse is mainlyN-glycolylneuraminic acid.Successive enzyme treatments with neuraminidase, papain, and trypsin reveal marked differences between the erythrocytes from various species.