Complete degradation of di-n-octyl phthalate by biochemical cooperation between Gordonia sp. strain JDC-2 and Arthrobacter sp. strain JDC-32 isolated from activated sludge.
Complete degradation of di-n-octyl phthalate by biochemical cooperation between Gordonia sp. strain JDC-2 and Arthrobacter sp. strain JDC-32 isolated from activated sludge.
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DOI：
10.1016/j.jhazmat.2009.11.022
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发表时间：
2010-04
影响因子：
13.6
通讯作者：
Xue-ling Wu;R. Liang;Qin-yun Dai;D. Jin;Yangyang Wang;W. Chao
中科院分区：
文献类型：
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作者：
Xue-ling Wu;R. Liang;Qin-yun Dai;D. Jin;Yangyang Wang;W. Chao
从以邻苯二甲酸酯（PAEs）混合物为唯一碳源和能源的活性污泥中分离到两株细菌。通过16 S rRNA基因序列分析，将其中一株菌鉴定为戈登氏菌JDC-2，另一株菌鉴定为节杆菌JDC-32。Gordonia sp.菌株JDC-2能快速降解邻苯二甲酸二辛酯（DOP），生成邻苯二甲酸（PA），并在培养基中积累。节杆菌属菌株JDC-32降解PA，但不降解DOP。Gordonia sp.菌株JDC-2和Arthrobacter sp.菌株JDC-32的共培养物通过克服各自单独的降解限制而完全降解DOP。通过对降解中间产物的分析，提出了戈登氏菌JDC-2降解DOP的生化途径。结果表明，DOP的完全降解是通过从活性污泥中分离的不同微生物的生化合作。
Two bacterial strains were isolated from activated sludge using mixtures of phthalic acid esters (PAEs) as the sole source of carbon and energy. One of the isolates was identified as Gordonia sp. strain JDC-2 and the other as Arthrobacter sp. strain JDC-32, mainly through 16S rRNA gene sequence analysis. Gordonia sp. strain JDC-2 rapidly degraded di-n-octyl phthalate (DOP) into phthalic acid (PA), which accumulated in the culture medium. Arthrobacter sp. strain JDC-32 degraded PA but not DOP. The co-culture of Gordonia sp. strain JDC-2 and Arthrobacter sp. strain JDC-32 degraded DOP completely by overcoming the degradative limitations of each species alone. The biochemical pathway of DOP degradation by Gordonia sp. strain JDC-2 was proposed based on the identified degradation intermediates. The results suggest that DOP is completely degraded by the biochemical cooperation of different microorganisms isolated from activated sludge.