Identification of the gene (lgtG) encoding the lipooligosaccharide β chain synthesizing glucosyl transferase from Neisseria gonorrhoeae
Identification of the gene (lgtG) encoding the lipooligosaccharide β chain synthesizing glucosyl transferase from Neisseria gonorrhoeae
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DOI：
10.1073/pnas.95.18.10872
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发表时间：
1998-09-01
影响因子：
11.1
通讯作者：
Stein, DC
中科院分区：
文献类型：
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作者：
Banerjee, A;Wang, R;Stein, DC
淋病奈瑟氏菌（GC）的脂寡糖由脂质A、寡糖核心和三个分支（α、β和γ）组成。我们报告的基因（lgtG，脂寡糖糖基转移酶G）编码的葡萄糖基转移酶的GC启动β链，其中包括乳糖基部分的克隆。该基因含有胞苷[poly（C）]的同聚体，我们证明了poly（C）中Cs数量的变化是不同GC菌株中β链表达变化的原因。生物化学分析和质谱法清楚地将mAb 2C 7的反应性归因于乳糖基β链的存在。此外，我们证明在不存在乳糖基的情况下，添加磷酸乙醇胺以产生新的抗原表位，如通过获得与mAb 2-L1-8的反应性所证明的。这些结果表明，与α链一样，脂寡糖的β链也会发生抗原变异。
The lipooligosaccharide from Neisseria gonorrhoeae (GC), consists of lipid A, an oligosaccharide core and three branches, alpha, beta, and gamma. We report the cloning of the gene (lgtG, lipooligosaccharide glycosyl transferase G) encoding the glucosyl transferase of GC that initiates the beta chain which consists of a lactosyl moiety. This gene contains a homopolymeric tract of cytidine [poly(C)] and we demonstrate that changes in the number of Cs in poly(C) account for the variation of beta chain expression in different GC strains. Biochemical analyses and mass spectrometry clearly attribute the reactivity of mAb 2C7 to the presence of the lactosyl beta chain, In addition, we demonstrate that in the absence of the lactosyl group, a phosphoethanolamine is added to generate a new antigenic epitope as evidenced by the gain of reactivity to mAb 2-L1-8. These results show that, like the alpha chain, the beta chain of lipooligosaccharide is subject to antigenic variation.