O-Specific Polysaccharide-Specific Memory B Cell Responses in Young Children, Older Children, and Adults Infected with Vibrio cholerae O1 Ogawa in Bangladesh
O-Specific Polysaccharide-Specific Memory B Cell Responses in Young Children, Older Children, and Adults Infected with Vibrio cholerae O1 Ogawa in Bangladesh
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DOI：
10.1128/cvi.00647-15
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发表时间：
2016-05-01
影响因子：
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通讯作者：
Ryan, Edward T.
中科院分区：
文献类型：
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作者：
Aktar, Amena;Rahman, M. Arifur;Ryan, Edward T.
霍乱弧菌引起的霍乱O 1赋予至少3至10年的保护，对随后的疾病，无论年龄，尽管在外周血中的抗体水平相对迅速下降，这表明记忆B细胞反应可能发挥重要作用的保护。脂多糖（LPS）的霍乱弧菌O 1特异性多糖（OSP）成分负责血清群特异性，目前尚不清楚，如果年幼的儿童能够发展记忆B细胞对OSP的反应，T细胞非依赖性抗原，霍乱。为了解决这一问题，我们评估了在孟加拉国达卡由霍乱弧菌O 1引起的霍乱中，幼儿（2至5岁，n = 11）、大龄儿童（6至17岁，n = 21）和成人（18至55岁，n = 28）的OSP特异性记忆B细胞反应。我们还评估了记忆B细胞对LPS的应答和杀弧菌应答，以及在恢复后第2天和第7天以及第30天、第90天和第180天对OSP、LPS和霍乱毒素B亚基（Ctx B; T细胞依赖性抗原）的血浆抗体应答。在所有年龄组中，杀弧菌应答和血浆OSP、LPS和CtxB特异性应答在第7天达到峰值，并在随访期间下降至基线。相比之下，我们能够在所有年龄组的患者中检测到OSP记忆B细胞应答，这些患者在90至180天的基线上具有可检测的应答。我们的研究结果表明，OSP特异性记忆B细胞反应可以发生在霍乱后，即使在最年幼的儿童，并可能部分解释年龄无关的诱导长期免疫自然获得性疾病。
Cholera caused by Vibrio cholerae O1 confers at least 3 to 10 years of protection against subsequent disease regardless of age, despite a relatively rapid fall in antibody levels in peripheral blood, suggesting that memory B cell responses may play an important role in protection. The V. cholerae O1-specific polysaccharide (OSP) component of lipopolysaccharide (LPS) is responsible for serogroup specificity, and it is unclear if young children are capable of developing memory B cell responses against OSP, a T cell-independent antigen, following cholera. To address this, we assessed OSP-specific memory B cell responses in young children (2 to 5 years, n = 11), older children (6 to 17 years, n = 21), and adults (18 to 55 years, n = 28) with cholera caused by V. cholerae O1 in Dhaka, Bangladesh. We also assessed memory B cell responses against LPS and vibriocidal responses, and plasma antibody responses against OSP, LPS, and cholera toxin B subunit (CtxB; a T cell-dependent antigen) on days 2 and 7, as well as days 30, 90, and 180 after convalescence. In all age cohorts, vibriocidal responses and plasma OSP, LPS, and CtxB-specific responses peaked on day 7 and fell toward baseline over the follow-up period. In comparison, we were able to detect OSP memory B cell responses in all age cohorts of patients with detectable responses over baseline for 90 to 180 days. Our results suggest that OSP-specific memory B cell responses can occur following cholera, even in the youngest children, and may explain in part the age-independent induction of long-term immunity following naturally acquired disease.